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RTv Reverse Transcriptase (Glycerol-free) is obtained from the M-MLV Reverse Transcriptase by directed genetic engineering, which has excellent reverse transcription efficiency, specificity, sensitivity and thermal stability. It is a lyophilizable version of RTv Reverse Transcriptase (Vazyme #RV101), applicable for Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP). With the new generation of hot start technology, the reaction system can be prepared at room temperature. Moreover, the enzyme activity is inhibited at temperatures below 45°C, thus improving specificity.