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During conventional PCR analysis, DNA fragments with high GC content are often difficult to amplify due to their stable secondary structure. Under ordinary PCR reaction conditions, DNA polymerase is difficult or even impossible to amplify.
It can intervene in the secondary structure of high GC content DNA. PCR Enhancer is a mixed additive composed of multiple components, which can effectively reduce the melting temperature of high GC templates and templates with complex secondary structures, and is compatible with almost all DNA polymerase amplification systems. When the optimized PCR program cannot effectively amplify the target fragment, adding PCR Enhancer can often get unexpected results.
Increase amplification stability;
Improve amplification efficiency
Store at -20°C for up to 18 months.