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One-LiteTM Luciferase Assay system is a highly sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity Luciferin and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed One-LiteTM detection reagent to the cell culture to lyse the cells and release the luciferase, then the reaction as shown in the figure can be generated and a stable light signal can be emitted.
Long-term storage: -30 ~ -15℃ for up to 36 months, transportation conditions: ≤0℃.
Before mixing: One-Lite Luciferase Assay Buffer can be stored at room temperature for 90 days (> 90% activity) or long-term storage at 2 ~ 8℃;
One-Lite Luciferase Assay Substrate can be stored at room temperature for 21 days or at 2 ~ 8 ℃ for 90 days (> 85% activity).
After mixing: One-LiteTM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 5 days (> 85% activity).
It can be kept stable even after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 60 days. If not used for a long time, it is recommended to store at -70°C.