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Mut Express MultiS Fast Mutagenesis Kit V2 C215

Mut Express MultiS Fast Mutagenesis Kit V2 C215

SKU: #001 - In Stock
USD$0.00

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Product Introduction

Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the possibility of introducing new mutations during the amplification process. Its excellent long-fragment amplification capability is widely applicable to the amplification of any plasmid with a length less than 20 kb. ClonExpress® rapid cloning System utilizes efficient homologous recombination The reaction replaces the traditional annealing reaction. Therefore, use Mut Express® MultiS site-directed mutagenesis kit for DNA site-directed In the case of mutation, the primer design is more flexible, and the amplification reaction is no longer performed in a linear manner, and the template usage is extremely low, which is beneficial to the original Complete degradation of the basic template. The kit is equipped with Exnase® MultiS, a recombinase optimized for multi-base site-directed mutagenesis, And if the amplified product is specific, the DpnI digested product can be used directly in the recombination reaction without DNA purification. Highly optimized reaction buffer Mut Express® MuiltiS Fast Mutagenesis Kit V2 has become the preferred kit for DNA multipoint mutations.

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Product Advantages

Phanta® Max Super-Fidelity DNA Polymerase provides high-fidelity PCR with the lowest mutation rate;
Phanta® Max Super-Fidelity DNA Polymerase has excellent long fragment amplification ability, which is widely applicable to the amplification of any plasmid within 20 kb;
The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template;
ClonExpress® rapid cloning system efficiently circularizes PCR products;
DpnI eliminates original template contamination
Site-directed mutagenesis of three to five discrete sites (more than 50 bp apart) on the target plasmid can be achieved in one step

Components

C215 Components

Storage

Store the product at -20℃, and it will be valid for 1 year.

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