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KeyPo DNA Polymerase is a new generation of high-fidelity DNA polymerase based on the directed evolution of KOD DNA Polymerase. It maintains ultra-high amplification success rate and sensitivity, and can achieve rapid amplification of 5 sec/kb. This product is widely applicable to conventional templates, GC-rich systems (primers or templates) and uracil-containing templates. By adding two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, it can perform the hot start PCR with great specificity. This product is a 2 × premix, which only needs to add templates and primers to peform amplification reaction, thereby simplifing operations. Amplification products are blunt-ended, which are compatible with Homologous Recombination kits (Vazyme #C112/C113/C115/C601) and TOPO cloning kits (Vazyme #C601).
Components | PK511-01 | PK511-02 | PK511-03 |
2 × KeyPo Master Mix (Dye Plus) | 1 ml | 5 × 1 ml | 15 × 1 ml |
Store at -30 ~ -15℃ for up to 12 months, and transport at ≤0℃.