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HiScript III U+ One Step qRT-PCR Probe Kit Q225

HiScript III U+ One Step qRT-PCR Probe Kit Q225

SKU: #001 - In Stock
  • Q225-01: Sales Specification: 100 rxns (30 μl/rxn)
  • Q225-02: Sales Specification: 1,000 rxns (30 μl/rxn)
  • Q225-03: Sales Specification: 5,000 rxns (30 μl/rxn)
  • USD$0.00

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    Product Description

    HiScript III U+ One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit contains a dUTP/UDG system that can prevent the contamination of PCR product. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III U+ One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. HiScript III U+ One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step U+ Mix contains an optimized buffer and dNTP/dUTP Mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).

    Product Features

    1. More sensitive amplification that is suitable for DNA and RNA templates
    2. More stable amplification curve with low concentration template
    3. Better storage conditions
    4. It supports rapid procedures and is suitable for rapid reactions

    Components

    Q225 Components

    Storage

    Store at -30 ~ -15℃ and transport at ≤0℃.

    Citation

    Li, Huiying, et al. "Mir-484 contributes to diminished ovarian reserve by regulating granulosa cell function via YAP1-mediated mitochondrial function and apoptosis." International Journal of Biological Sciences 18.3 (2022): 1008-1021.Impact Factor:6.582

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