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HiScript III U+ One Step qRT-PCR Probe 5× Master Mix Q611

HiScript III U+ One Step qRT-PCR Probe 5× Master Mix Q611

SKU: #001 - In Stock
  • Q611-01: Sales Specification: 100 rxns
  • Q611-02: Sales Specification: 1000 rxns
  • Q611-03: Sales Specification: 10000 rxns
  • USD$0.00

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    Product Description

    HiScript III U+ One Step qRT-PCR Probe 5 × Master Mix is specially designed for single-plex or multiplex qPCR detection using RNA (e.g. virus RNA) as template. It has extremely high stability, which can be stored at 37℃ for 14 days and its performance remains unchanged, and remains liquid form at a storage temperature of - 20℃ to avoid the thawing process of reagents. The reaction can be started by directly adding the template, primers and target probes required for detection. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit contains a dUTP/UDG system that can prevent the contamination of PCR product. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III U+ One Step qRT-PCR Probe 5 × Master Mix can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).

    Product Features

    1. Using gene specific primers (GSP), the reverse transcription and
    qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination
    2. More sensitive amplification that is suitable for DNA and RNA templates
    3. More stable amplification curve with low concentration template
    4. Better storage conditions
    5. It supports rapid procedures and is suitable for rapid reactions

    Components

    Q611 Components

    Storage

    Store at -30 ~ -15℃ and transport at ≤0℃.

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