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HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) R312

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) R312

SKU: #001 - In Stock
  • R312-01: Sales specifications: 50 rxn
  • R312-02: Sales specifications: 100 rxn
  • USD$0.00

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    Storage Conditions

    Store at -30~-15°C (restore 10*RT Mix to room temperature and shake well for use)

    Product Introduction

    HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.

    Product Advantages

    Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product;
    Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs;
    gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns

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