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HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.
Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product;
Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs;
gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns
Store at -30 ~ -15℃ and transport at ≤0℃.
10 × RT Mix contains high concentration of DTT, which may precipitate at low temperatures. Please restore to room temperature, shake gently and mix thoroughly, wait to dissolve the precipitation again before use.
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