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HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) R312

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) R312

SKU: #001 - In Stock
  • R312-01: Sales specifications: 50 rxn
  • R312-02: Sales specifications: 100 rxn
  • USD$0.00

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    Product Detail

    Application Notes

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    Product Introduction

    HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.

    Product Advantages

    Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product;
    Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs;
    gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns


    R312 Components


    Store at -30 ~ -15℃ and transport at ≤0℃.

    10 × RT Mix contains high concentration of DTT, which may precipitate at low temperatures. Please restore to room temperature, shake gently and mix thoroughly, wait to dissolve the precipitation again before use.


    Wang, Le-Yun, et al. "Overcoming intrinsic H3K27me3 imprinting barriers improves post-implantation development after somatic cell nuclear transfer." Cell stem cell 27.2 (2020): 315-325.Impact Factor:24.633

    Chen, Baiwen, et al. "DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion." Nature communications 13.1 (2022): 1-14.Impact Factor:14.919

    Ma, Xiao-Ru, et al. "Restoring nuclear entry of Sirtuin 2 in oligodendrocyte progenitor cells promotes remyelination during ageing." Nature communications 13.1 (2022): 1-19.Impact Factor:14.919

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