• day ::

This product was successfully added to cart!

View Shopping Cart

HiScript II U+ One Step qRT-PCR Probe Kit Q223

HiScript II U+ One Step qRT-PCR Probe Kit Q223

SKU: #001 - In Stock
USD$0.00

Please note that pricing may vary due to different countries and regions
The Price Does Not Include Taxes and Shipping Fees

Request Contact

Click and submit your question and we will get back to you within one business day

Product Detail

Application Notes

Customer Reviews

Product Tags

Product Introduction

The dUTP/UDG anti-contamination system is a very effective means to control the contamination of PCR amplification products. In PCR detection reagents using DNA as a template, it has gradually become a mandatory standard. However, the commonly used E. coli UDG It still has high activity at the usual reverse transcription temperature (42-55℃), which will degrade cDNA, which will reduce the sensitivity of One Step RT-PCR/qPCR.
HiScript® II U+ One Step qRT-PCR Probe Kit contains an optimized ratio of dUTP/dNTP mix and heat-labile UDG derived from psychrophilic marine bacteria. Heat-labile UDG has high activity at room temperature and can fully degrade U-containing double-stranded DNA during the mixing process of the reaction system. When the reaction system is heated to 50-55°C (the optimal reaction temperature of HiScript® II), Heat-labile UDG is quickly and completely inactivated, maintaining the integrity of the cDNA, and ensuring that the detection sensitivity is not affected.

Product Advantages

Support multiple probe detection;
UDG anti-pollution system: Heat-labile UDG rapidly degrades U-containing double-stranded DNA contaminants at room temperature. Heat-labile UDG is rapidly and completely inactivated at 50-55°C to maintain the integrity of cDNA;
Ultra-high detection sensitivity: single-digit copies of templates or 0.1 pg total RNA can be detected
Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;

Components

q223 COMPONENTS

Storage

Stored at -20℃ for up to 18 months.

Cat. No.:
--- Please Select ---

Application Notes Abstract

Read More

Write a review here:

  • 1 Star2 Stars3 Stars4 Stars5 Stars (No Ratings Yet)
    Loading ... Loading ...

  • 7 × = fifty six

    Tags: , , , , , , , , , , , , , ,

    Customers Also Bought: