Click and submit your question and we will get back to you within one business day
HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for two-step qRT-PCR. The 5 × HiScript qRT SuperMix contains all components required for reverse transcription. With the addition of template RNA and ddH2O, the reaction can proceed quickly. The volume of template RNA can be up to 80% of the total volume, which is very suitable for reverse transcription reaction at low concentration of template RNA. 5 × HiScript II qRT SuperMix will not freeze at -20℃.
This product has been specially optimized for qPCR. Oligo (dT)23VN has stronger anchoring ability for poly A+ mRNA than Oligo (dT)18, resulting in higher reverse transcription efficiency. With the optimized proportion of Random primers/Oligo (dT)23VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results. The RT product is compatible with dye-based and probe-based qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.
Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher;
Anchored Oligo (dT)23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis;
The ready-to-use SuperMix is easy to use and time-saving
Store at -30 ~ -15℃ for up to 12 months, and transport at ≤0℃.