HiScript II Q RT SuperMix for qPCR R222

HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for two-step qRT-PCR. The 5 × HiScript qRT SuperMix contains all components required for reverse transcription. With the addition of template RNA and ddH₂O, the reaction can proceed quickly. The volume of template RNA can be up to 80% of the total volume, which is very suitable for reverse transcription reaction at low concentration of template RNA. 5 × HiScript II qRT SuperMix will not freeze at -20℃.
This product has been specially optimized for qPCR. Oligo (dT)₂₃VN has stronger anchoring ability for poly A⁺ mRNA than Oligo (dT)₁₈, resulting in higher reverse transcription efficiency. With the optimized proportion of Random primers/Oligo (dT)₂₃VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results. The RT product is compatible with dye-based and probe-based qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.