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HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved thermo stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR (+gDNA wiper) is specially designed for 2-step qRT-PCR. The residual genomic DNA in RNA template can be removed rapidly and completely with the 4 × gDNA Wiper Mix. The 5 × HiScript qRT SuperMix II contains all necessary components required for reverse transcription. With the addition of template RNA and ddH2O, the reaction can proceed quickly. Meanwhile, gDNA wiper is terminated to ensure the integrity of the cDNA. This product has been specially optimized for qPCR. With the optimized proportion of Random primers/Oligo (dT)23VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results to the greatest extent. The cDNA products are compatible with SYBR Green and probe qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.
Store at -30 ~ -15℃ for up to 12 months, and transport at ≤0℃.