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HiScript II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrate HiScript II Reverse Transcriptase and the superior performance of Champagne TaqTM plus DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system HiScript II One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of HiScript II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.
Reverse transcription and PCR are performed in the same tube;
The detection sensitivity can reach 0.1 pg total RNA;
Amplify long fragments over 10 kb
Store at -30 ~ -15℃ for up to 18 months, and transport at ≤0℃.