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HiScript II 1st Strand cDNA Synthesis Kit R211

HiScript II 1st Strand cDNA Synthesis Kit R211

SKU: #001 - In Stock
  • R211-01: Sales specifications: 50 rxn
  • R211-02: Sales specifications: 100 rxn
  • USD$0.00

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    Product Detail

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    Product Introduction

    HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.

    Product Advantages

    Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA;
    A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more;
    Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis;
    Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.

    Components

    R211 Components

    Storage

    Store at -30 ~ -15℃ and transport at ≤0℃.

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