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HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology based on M-MLV (RNase H-) Reverse Transcriptase. Compared with HiScript Reverse Transcriptase, HiScript II further improves the thermal stability and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript II adds several point mutations that further enhances template binding affinity and cDNA synthesis efficiency, and has higher tolerance to common reverse transcription inhibitors.
This product is 1st strand cDNA synthesis kit based on HiScript II Reverse Transcriptase. It contains all the components necessary for high quality first strand cDNA synthesis, and the product is suitable for subsequent PCR, qPCR and other experiments. The 2 × RT Mix contains an optimized buffer and dNTPs. The HiScript II Enzyme Mix contains the HiScript II Reverse Transcriptase and the RNase inhibitor. Oligo (dT)23VN has stronger anchoring ability for poly A+ mRNA than Oligo (dT)18, which makes reverse transcription more efficient. Oligo (dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. The kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.
Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA;
A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more;
Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis;
Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.
Store at -30 ~ -15℃ for up to 18 months, and transport at ≤0℃.