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This kit can quickly extract total RNA from animal tissues and cells. The kit is based on silica gel column purification technology. There is no need to use toxic phenol/chloroform extraction or β-mercaptoethanol to extract high-quality RNA during the extraction process. The whole process only takes 6 minutes. The FastPure gDNA-Filter Columns Ⅲ in the kit can effectively remove impurities and gDNA, and FastPure RNA Columns Ⅲ can efficiently bind RNA. With optimized buffer, the total RNA obtained is high in purity, less gDNA residues, and free of protein and other impurities. It can be used for various downstream experiments such as RT-PCR, Real-Time PCR, chip analysis, etc.
Safe and non-toxic: no need for β-mercaptoethanol and phenol/chloroform
Fast: 6min operation at room temperature
Few genome residues: no need to digest on DNase I membrane, remove gDNA contamination in one step
High RNA purity: The obtained RNA can be directly used in downstream experiments with high purity requirements
Strong versatility: compatible with various cell and tissue samples
Except for Buffer AO, the other components of this product are stored at room temperature (15 ~ 25; transportation at room temperature).
Buffer AO can be stored at 15 ~ 25 ℃ for one month, long-term storage needs to be placed at 20 room temperature for transportation.