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The Equalbit RNA BR (Broad-Range) Assay Kit is a simple, sensitive, and accurate RNA fluorescence quantitative detection kit. This kit contains fluorescence detection reagents, buffers, and dsDNA standards. This kit is highly selective for RNA and is not subject to dsDNA. It has an excellent linearity between 20 ng and 1000 ng for RNA samples, providing accurate quantification of total RNA, rRNA, mRNA samples from 1 ng /μl to 1000 ng /μl. This kit has excellent resistance for most conventional pollutants, including salt, free nucleotides, proteins, solvents, and detergents. The Equalbit RNA BR (Broad-Range) Assay Kit is easy to operate, and the assay can be performed at room temperature. Before use, please dilute the fluorescence detection reagent with buffer into a working solution, and then add the RNA sample for detection by a Qubit® fluorometer.
The complete kit should be stored at 2~8℃, protected from light; after the first use, it is recommended to store Equalbit dsDNA HS Reagent at room temperature and protect from light; Equalbit dsDNA HS Buffer should be stored at room temperature; Equalbit dsDNA HS Standard #1 and #2 should be stored at 4℃
Precise sensitivity: It can accurately quantify total RNA, rRNA, and mRNA samples with a concentration of 250 pg/µl to 100 ng/µl;
Ultra-high specificity: This product is highly selective for RNA, not affected by DNA, and is excellent for some conventional pollutants, such as salt, free nucleotides, proteins, solvents, detergents, etc. Tolerance
Operational simplicity: This product is easy to operate. The fluorescence detection reagents are diluted with buffer solution into working solution, and then the RNA sample to be tested is added to the Qubit® Fluorometer for detection. This operation can be performed at room temperature.