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This product is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component Champagne Taq DNA Polymerase is a new type of antibody-based hot-start DNA polymerase, which has many advantages such as strong specificity and high detection sensitivity. It is equipped with the most suitable buffer optimized for qPCR and the patented specific promoter Exactor, which is very suitable for high specificity and high sensitivity qPCR. This product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments, so that no need to adjust the concentration of ROX on different instruments, only need to add primers and templates when preparing the reaction system to perform amplification.
Wide platform applicability: special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments;
Ultra-high amplification specificity: the use of a new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patent specific promotion factor ExactorTM, effectively avoid the generation of primer dimers and non-specific amplification;
Excellent amplification sensitivity: The master mix has an excellent linear relationship in a wide template range, and can detect single-digit copies of the template to be tested;
Excellent program compatibility: Standard programs and fast programs can be used to quantify the premix, which perfectly solves program compatibility.
Store at -30 ~ -15℃ and protect from light for up to 18 months. Master mix can be stored stably at 2 ~ 8℃ for 6 months and protected from light after thawing. Transport at ≤ 0℃.
▲After the master mix is thawed, a little white precipitation may appear. Please place it at room temperature for a while and turn it up and down to dissolve the precipitate. Please confirm that the precipitation is completely dissolved and fully mixed before use.