Bradford Protein Quantification Kit E111

Bradford method (Coomassie brilliant blue method) is one of the most sensitive methods for determining protein concentration. When Bradford staining solution (Coomassie Brilliant Blue G250) and protein are combined under acidic conditions, the color of the solution changes from brown-black to blue, and the maximum absorbance wavelength changes from 456 nm to 595 nm. The absorbance and protein content are at a certain concentration. There is a good linear relationship within the range. By measuring the absorbance value and comparing the absorbance value of the standard protein, the protein concentration is calculated, and the protein concentration determination is fast, stable and highly sensitive. Bradford staining solution is a 2 times concentrated mother liquor for easy storage.