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Blood Direct PCR Kit can perform PCR directly of whole blood samples without the need for DNA purification or sample pretreatment. This kit can be used to perform PCR directly from a wide range of templates including fresh blood, blood stored at 4°C, frozen blood, and dried blood stored on Whatman 903 or FTA commercial card. It is compatible with all conventional anticoagulants (EDTA, citrate, heparin, etc.). The Kit contains the Phanta Blood Super-Fidelity DNA Polymerase, which is an engineering modification of the Phanta Blood Super-Fidelity DNA Polymerase. This enzyme displays superior resistance to the PCR inhibitors in the whole blood sample, and can amplify whole blood with concentration up to 40%. This kit can efficiently amplify genomic fragments of up to 10 kb from whole blood samples with the optimized 2 × Phanta Blood Buffer V2.
Phanta Blood Super-Fidelity DNA Polymerase produces blunt-ended products, which are applicable to ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). This kit supplies Positive control primer mix that is compatible with mammalian and other vertebrate animals and can be used as positive control in experiments.
Special lysate based on proteinase K;
The lysate is directly used for PCR amplification without the need to extract the genome;
It is widely suitable for the amplification of target fragments within 2 kb, and is suitable for multiple PCR reactions within 4 pairs of primers.
Store at -30 ~ -15℃ for up to 18 months, and transport at ≤0℃.