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RNA Isolater Total RNA Extraction Reagent is stored at 4℃ and protected from light; Bacteria RNA Plus Reagent is stored at room temperature
Bacteria RNA Extraction Kit is a kit optimized for RNA extraction from prokaryotes (including Gram-negative and Gram-positive bacteria). This kit includes Bacteria RNA Plus Reagent containing optimized buffer system, chelating agent and mild protein denaturant, and RNA isolater Total RNA Extraction Reagent that can effectively inhibit endogenous RNase and promote protein denaturation. The combination of the two can greatly increase the yield of prokaryotic RNA extraction while ensuring good product quality. When extracting bacterial RNA, only one step of high temperature pretreatment is added before routine operations, without mechanical disruption or enzymatic digestion steps, and high-quality and yielding bacterial total RNA can be obtained. This kit is also suitable for extraction of total RNA from yeast and other fungi.
Targeted optimization for the extraction of total RNA from prokaryotes (gram-positive bacteria and gram-negative bacteria) and fungi (non-spores);
The pretreatment solution is used in conjunction with the guanidine isothiocyanate-based lysis solution to greatly increase the RNA yield and purity;
Simple operation, time saving, no need for lysozyme or ultrasonic treatment