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AceQ Universal SYBR qPCR Master Mix is a special premix for qPCR detection using SYBR Green I chimeric fluorescence method. The core component AceTaq DNA Polymerase is a chemically modified hot-start DNA polymerase. Combined with the optimal Buffer optimized for qPCR, nonspecific amplification can be effectively inhibited, thus significantly improving the amplification efficiency, which is suitable for high-sensitivity qPCR detection. This product is a dye-based 2 × master mix. A good standard curve can be obtained in a wide quantitative area, and the target gene can be accurately quantified and detected, with good repeatability and high reliability. This product contains a unique ROX Passive Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.