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5min Universal Ligation Mix is a ready-to-use 2 × premix. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphodiester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized ligation enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can be directly transformed into a variety of chemically competent cells.
Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection;
Fast: 25℃, 5 minutes can be connected quickly;
High efficiency: High cloning efficiency.
Store at -30 ~ -15℃ for up to 12 months. Transportation conditions: ≤0℃.