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This product is the third generation TOPO cloning kit, which contains Topoisomerase, vector and self-ligation inhibitor. The kit uses a new type of Topoisomerase, with the optimal buffer, the enzyme activity is higher, and the high-efficiency ligation can be completed in 5 minutes. The addition of a vector self-ligation inhibitor increases the positive rate of clones. This product is suitable for blunt-end cloning.