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Phanta Max Super-Fidelity DNA Polymerase is a new generation superior enzyme based on Phanta DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau un-inhibitory factor newly added to Phanta Max greatly improve its long-fragment amplification ability, specificity, and PCR yield. Phanta Max is capable of amplifying long fragments such as 40 kb λDNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibit- ing the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable Phanta Max to perform hot start PCR with great specificity. This kit contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. It contains all required reaction components, except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Protective agents in the 2 × Phanta Max Master Mix enable the resistance to repeated freezing and thawing. Amplification will generate blunt-ended products, which are compatible with ClonExpress and Top Cloning kit (Vazyme #C112/C113/C115/C601).
Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches;
Faster amplification: The limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments;
Longer amplification: The effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb;
Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples;
Simple operation: Dye premix, minimize experimental steps.
1. Excellent high GC amplification capacity
The 654 bp, 900 bp, 800 bp, 1,200 bp, 1,400 bp and 426 bp target fragments were amplified using the human genome as a template, and the GC content of each amplicon was higher than 68%.
2. Trusted amplification fidelity
3. Stable crude amplification ability
|2 × Phanta Max Master Mix||1 ml||5 × 1 ml||15 × 1 ml|
Store at -30 ~ -15℃ and transport at ≤0℃.
▲ Avoid repeated freezing and thawing.
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