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2 × Taq Plus Master Mix Ⅱ (Dye Plus) P213
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Product Introduction
2 × Taq Plus Master Mix II (Dye Plus) contains Taq Plus DNA Polymerase, dNTP and an optimized buffer system, which is suitable for high yield PCR. Compared with Taq DNA Polymerase, it has higher fidelity, stronger amplification performance and higher yield. This product can efficiently amplify up to 10 kb genomic DNA fragments, 15 kb plasmid DNA fragments and λDNA fragments. The 2 × Taq Plus Master Mix II only needs to add primers and templates to perform amplification reaction, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The system contains protective agents that keep 2 × Taq Plus Master Mix II stable in activity after repeated freezing and thawing. This product containing loading buffer, so PCR products can be directly loaded for electrophoresis after the reaction, which is convenient to use. The PCR product has an adenine at the 3' end that can be cloned into the T vector. It is also compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601).
Product Advantages
Add 3'→5' exonuclease activity;
Fidelity is 6 times that of Taq DNA Polymerase;
Improve amplification yield and length;
Ready-to-use premix, minimizing experimental steps
Components
Storage
Store at -30 ~ -15℃ for up to 12 months, and transport at ≤0℃.
Citation
Yu, Yuanhuan, et al. "Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors." Science advances 6.28 (2020): eabb1777.
Wang, Xinyi, et al. "A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation." Science advances 7.50 (2021): eabh2358.
Sheng, Dandan, et al. "Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization." Journal for ImmunoTherapy of Cancer 10.5 (2022): e003793.
He, Lingli, et al. "A regulation loop between YAP and NR4A1 balances cell proliferation and apoptosis." Cell Reports 33.3 (2020): 108284.
Liang, Li-Mei, et al. "Splicing factor SRSF6 mediates pleural fibrosis." JCI insight 6.10 (2021).
Application Notes Abstract
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2 × Taq Plus Master Mix (Dye Plus) P212
