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2 × Taq Plus Master Mix II (Dye Plus) P213
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Product Detail
Application Notes
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Product Introduction
This product contains Taq Plus DNA Polymerase, dNTP and an optimized buffer system. It can be amplified by adding primers and templates, which reduces pipetting operations and improves throughput and results reproducibility. The protective agent added to the system allows Master Mix to maintain stable activity after repeated freezing and thawing. This product is available in versions containing running buffers and loading dye for direct electrophoresis after the reaction. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress rapid cloning kit (C112/C113).
Product Advantages
Add 3'→5' exonuclease activity;
Fidelity is 6 times that of Taq DNA Polymerase;
Improve amplification yield and length;
Ready-to-use premix, minimizing experimental steps
Components
Storage
Store at -30 ~ -15℃ for up to 12 months, and transport at ≤0℃.
Citation
Yu, Yuanhuan, et al. "Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors." Science advances 6.28 (2020): eabb1777.
Wang, Xinyi, et al. "A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation." Science advances 7.50 (2021): eabh2358.
Sheng, Dandan, et al. "Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization." Journal for ImmunoTherapy of Cancer 10.5 (2022): e003793.
He, Lingli, et al. "A regulation loop between YAP and NR4A1 balances cell proliferation and apoptosis." Cell Reports 33.3 (2020): 108284.
Liang, Li-Mei, et al. "Splicing factor SRSF6 mediates pleural fibrosis." JCI insight 6.10 (2021).
Application Notes Abstract
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2 × Taq Plus Master Mix (Dye Plus) P212
