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This product contains Taq DNA Polymerase, extension promoter, dNTP and optimized buffer system. The amplification speed can reach 15 sec/kb. It is suitable for rapid PCR reaction. The limit amplification speed within 1 kb can reach 1 sec/kb, which is large Save PCR reaction time. The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. This product has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb using genome as template and PCR amplification within 10 kb using plasmid and λDNA as template. The protective agent added to the amplification system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. This product contains electrophoresis buffer and green dye. It can be directly electrophoresed after the reaction, which is convenient to use. The PCR product has an A at the 3’ end, which can be directly cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114)
Fast amplification speed: 15 sec/kb, the limit amplification speed can reach 1 sec/kb within 1 kb;
Convenient operation: amplification can be carried out by adding primers and templates, no operation on ice is required, and the product containing dye can be directly subjected to electrophoresis;
Good stability: repeated freezing and thawing for 50 times, no significant decrease in activity
Store at -20℃ for up to 18 months; can be stored at 4℃ for 4 months after thawing
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Cao, Qiqi, et al. "The Recurrent Mutation in PATL2 Inhibits Its Degradation Thus Causing Female Infertility Characterized by Oocyte Maturation Defect Through Regulation of the Mos-MAPK Pathway." Frontiers in cell and developmental biology (2021): 116.