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What is the Magic of High-Fidelity DNA Polymerase?

  • Time of issue:2021-07-28 16:46

(Summary description)In order to help you in completing PCR experiments better and faster, Vazyme is pleased to introduce fast high-fidelity polymerase. Check by cliking links below  P510 https://lnkd.in/dqd95P8  P520 https://lnkd.in/dVUSCrT

What is the Magic of High-Fidelity DNA Polymerase?

(Summary description)In order to help you in completing PCR experiments better and faster, Vazyme is pleased to introduce fast high-fidelity polymerase.
Check by cliking links below
 P510 https://lnkd.in/dqd95P8
 P520 https://lnkd.in/dVUSCrT

  • Categories:News
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  • Time of issue:2021-07-28 16:46
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     High-fidelity DNA polymerase contains a polymerase domain and a exonuclease domain. The polymerase domain has 5’-3’polymerase activity and is responsible for polymerization; the exonuclease domain has 3’-5’exonuclease activity (also called proofreading activity) and is responsible for removing mismatched nucleotides.
     In the process of PCR amplification, when the primer is fully complementary to the template, it enters the polymerase domain. Under the action of polymerase activity, the free deoxynucleotides are sequentially bound to the 3’end of the primer under the principle of complementary paring, and the new strand DNA extends from 5’end to 3’end . When the nucleotide bound to the primer terminus does not match the template, the mismatched nucleotide will be lifted and the new strand DNA can’t continue to extend, thus entering the exonuclease domain. Under the action of exonuclease activity, the mismatched nucleotide will be excised. After excision, this site will be bound to the correct nucleotide, so that the new strand DNA can continue to extend from 5’end to 3’end, which enables the new strand DNA to be replicated precisely.


Application:

Gene function research
Genetic sequencing
High-fidelity DNA polymerase can also be used in genotyping.
 
In order to help you in completing PCR experiments better and faster, Vazyme is pleased to introduce fast high-fidelity polymerase.
Check by cliking links below
https://lnkd.in/dqd95P8
https://lnkd.in/dVUSCrT


 

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