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Bulletin |“Monkeypox Diagnosis And Treatment Guide”- Vazyme Offers High-Quality Diagnostic Reagents

International Infection Status

According to the World Health Organization (WHO), from May 13, 2022 to June 8, 2022, Monkeypox has spread to the Americas, Eastern Mediterranean, Europe, and Western Pacific regions. Twenty-eight countries in the four regions have reported 1,285 confirmed cases and one suspected case to the World Health Organization. So far, no deaths have been reported in these four areas.

87% of these reported cases were from the European region (1112), as well as the Americas (153), the Eastern Mediterranean (14), and the Western Pacific (6). These four regions previously belonged to areas where Monkeypox is uncommon or where Monkeypox-related reports have never occurred.

Table 1

Table 1. Monkeypox cases reported to WHO in non-endemic countries between 13 May 2022 and 8 June 2022

Figure 1. Geographical distribution of Monkeypox cases reported from May 13 to June 8, 2022 at 5 p.m. (WHO) (Figure comes from https://www.who.int/emergencies/disease-outbreak-news/item/2022-DON392 [WHO:Multi-country Monkeypox outbreak: situation update])

From January 2022 to June 8, 2022, 1536 suspected cases and 72 deaths have been reported in 8 African countries. Six countries reported 59 confirmed cases during the same period.

Table 2

Table 2. Monkeypox cases reported to the World Health Organization in the African Region from January 2022 to 8 June 2022

The World Health Organization expects more Monkeypox cases to emerge as the surveillance area expands.

Monkeypox Diagnosis and Treatment Guide

The clinical presentation of cases in this Monkeypox outbreak varied. Common clinical manifestations of Monkeypox are fever, swollen lymph nodes, and a rash on the face and extremities. However, many cases in the current outbreak do not show the common clinical manifestations of Monkeypox, but only a few or single lesions.
Currently, laboratory testing methods for Monkeypox virus include General Testing and Etiological Testing:

1. General Testing

Peripheral blood leukocytes are normal or elevated, and platelets are normal or decreased. Some patients may have elevated transaminase levels, lower blood urea nitrogen levels, and hypoproteinemia.

2. Etiological Testing

(1) Nucleic acid detection: Monkeypox virus nucleic acid can be detected in specimens such as rash, blister fluid rash, blister fluid, crust, oropharyngeal or nasopharyngeal secretions.
(2) Virus culture: Monkeypox virus can be isolated by collecting the above specimens for virus culture.

Vazyme Solutions

Since the outbreak of Monkeypox, Vazyme has devoted to the research and development of products and solutions response to the epidemic. As a supplier of professional enzymes and solutions in the fields of life science, IVD and biomedicine, Vazyme can provide you with a complete set of solutions for product development including amplification reagent, positive parameter and primer probe design to help you quickly complete product development.

1. Sample Processing

Nucleic Acid Isolation Cat.No. Name Sample Types Details
Magnetic Bead Method RM411 Virus DNA/RNA Extraction Kit 2.0 (Prepackaged) Blood, serum, plasma, swab wash, fecal swab, etc.

14 min rapid extraction

Column-based Method RC313 FastPure® Viral DNA/RNA Mini Kit Pro Whole blood, serum, plasma, bronchoalveolar lavage fluid, nasopharyngeal or oropharyngeal swab, cultured cell supernatant, fecal swab, etc. Compatible; Rapid extraction

2. Nucleic Acid Diffusion (Click on the Cat.No. to see the detail)

Series Cat.No Name Details
Enzyme Mix qPCR mix QN213 Taq Pro U+ Multiple Probe qPCR Mix


QV114 Animal Detection U+ Probe qPCR Super PreMix

Support primer-probe full premix;
Compatible with rapid programs

Single Enzyme Hot-Start P132 Taq HS DNA Polymerase

Suitable for multiple reactions;

Heat-labile UDG P051 Heat-labile UDG

Universal anti-pollution matching system;
Rapid inactivation at 55°C;
Compatible with common PCR reaction systems

Monoclonal Antibody P121 Champagne Taq antibody

Thermal stable;
High purity;
Batch stable

3. Product Performance

Taq Pro U+ Multiple Probe qPCR Mix (Vazyme #QN213)

Figure 2. Amplification plot of Monkeypox detection

Vazyme #QN213 was used to detect Monkeypox plasmid samples with different concentration gradients (10, 1, 0.1, 0.01 fg, respectively). The amplification plot shows that the amplification results are uniform and the sensitivity is high.

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