High-quality, high-integrity DNA is the cornerstone of every successful cloning workflow. At Vazyme, we design our nucleic acid extraction solutions to deliver consistently pure results across a wide range of sample types—whether you're starting with delicate plasma or challenging, polysaccharide-rich plant tissues. With robust performance from the very first step, our kits let you focus on discovery, not troubleshooting.

For Blood/Cell/Tissue/Bacteria samples:

Whether you're working with mammalian cells, difficult tissues, or microbial samples, FastPure Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit (Vazyme #DC112) consistently delivers impressive DNA concentrations alongside excellent purity, enabling reliable performance in downstream applications such as qPCR, cloning, and sequencing. It also features high quality and good integrity, wide sample applicability, and a simple yet efficient workflow—making it an ideal choice for diverse experimental needs.

• High DNA yields and purity: 

The extracted DNA has the characteristics of high yield, good purity and complete fragments.

Genomic DNA was extracted from HEK 293 cells, rat liver tissue, and Staphylococcus aureus with Vazyme #DC112 ("V" in the figures), products of Suppliers A, B, C, and D, and similar products of Vazyme. The obtained DNA was detected for concentration and purity. It can be seen from the figures that for each sample type, Vazyme #DC112 extracts genomic DNA with a higher yield than the other similar products, and the OD260/280 and OD260/230 ratios are closer to the standard values, indicating better DNA purity.

For plant samples:

Plant genomic DNA extraction is often hindered by complex secondary metabolites and tough cell walls. FastPure Plant DNA Isolation Mini Kit (Vazyme #DC104) is designed for exceptional adaptability, efficiently processing a wide range of plant species—from herbaceous crops to woody plants—without compromising DNA integrity or yield. It offers a simple and fast protocol, broad application across diverse plant types, and consistently delivers high yield, good integrity, and high purity—making it an excellent tool for reliable plant DNA extraction.

• Broad compatibility: 

Compatible with different types of plant genomic DNA.

High-molecular-weight genomic DNA was extracted from nine different plant samples—including soybean, corn, rice, wheat, cotton, Rosa chinensis, and pine tree—using Vazyme’s FastPure Plant DNA kits. All lanes show clear, intact bands without significant degradation or contamination. Even challenging samples like Rosa chinensis and pine tree yielded high-integrity DNA, underscoring the kit’s versatility in agricultural and botanical genomics applications.

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When precision matters most, Vazyme’s high-fidelity PCR solutions deliver. Designed for applications that demand ultra-low error rates—such as cloning, mutagenesis, and next-generation sequencing—our high-fidelity mixes combine cutting-edge enzyme engineering with finely tuned buffer systems to ensure exceptional accuracy and efficiency. Even with complex or low-abundance templates, researchers can rely on robust amplification and minimal replication errors. From the very first cycle to the final result, Vazyme empowers molecular workflows with consistent, high-quality performance.

• Ultra-high fidelity

In high-fidelity PCR applications, the accuracy of DNA polymerases is crucial to ensuring trustworthy downstream results—whether in cloning, mutagenesis, or next-generation sequencing. According to a study based on next-generation sequencing (P. Mielinis et al., Journal of Molecular Biology, 2021), Phanta UniFi DNA Polymerase（Vazyme #P516）demonstrates an error rate that is 220 times lower than that of conventional Taq polymerase. This exceptional fidelity dramatically reduces the risk of amplification-induced mutations. As illustrated in the figure, Phanta outperforms comparable products from major suppliers, offering unmatched accuracy for demanding molecular workflows.

• Universal Compatibility and Robust Amplification

To evaluate thermal tolerance and workflow versatility, we conducted two sets of comparisons using 2× Phanta UniFi Master Mix (Vazyme #P516), 2× Phanta UniFi Master Mix (Dye Plus) (Vazyme #P526), and three leading high-fidelity polymerases from commercial suppliers (A, B, and C).

In the first experiment, DNA templates of varying sizes (1100 bp, 2000 bp, and 4800 bp) were amplified across a broad range of annealing temperatures (40–70 ℃). As shown in the gel images, both P516 and P526 consistently delivered strong, specific amplification across all conditions—demonstrating exceptional Tm adaptability and outperforming commercial alternatives, which showed variable efficiency and reduced yield.

In the second experiment, a fixed PCR program (Universal primer annealing at 60°C) was applied to amplify DNA fragments ranging from 313 bp to 10 kb, with primer Tm values spanning 56–64 °C. Both P516 and P526 succeeded in amplifying all targets with high yield and fidelity, without the need for condition-specific optimization.

Together, these results highlight the superior robustness, wide-range compatibility, and operational simplicity of Phanta UniFi mixes—making them ideal for high-throughput screening, multi-target workflows, and unpredictable or challenging PCR setups.

• Broad Compatibility for Diverse and Demanding PCR Needs

Phanta UniFi Master Mixes (P516 and P526) deliver consistently superior results across a wide array of demanding PCR scenarios. From ultra-long fragments—17.5 kb genomic DNA, 18.2 kb plasmid, 17.8 kb lambda DNA, to 15 kb cDNA—to GC-rich and GC-poor regions (ranging from 24% to 79% overall, and up to 100% locally), Phanta UniFi ensures reliable performance.

Even under challenging conditions, such as direct amplification from crude biological samples—including E. coli, Pichia pastoris, mouse tail lysate, rice tissue, and whole blood—Phanta UniFi maintained high yield and specificity without the need for extensive optimization.

Furthermore, Phanta UniFi supports up to 17-plex amplification for targets under 2 kb, making it an ideal choice for multiplex PCR applications. In all tested conditions, it consistently outperformed leading high-fidelity polymerases from Suppliers A, B, and C, showcasing its robustness, sensitivity, and unmatched versatility.

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Vazyme’s ClonExpress Ultra Kits bring seamless cloning to the next level—combining the reliability of traditional methods with a scarless, sequence-independent assembly approach. Whether you're performing routine cloning or constructing complex genetic circuits, our single-tube, one-step system enables fast and accurate insertion of one or multiple DNA fragments into virtually any plasmid backbone.

Designed for maximum compatibility across diverse vector types and fragment sizes, ClonExpress Ultra minimizes hands-on time while maximizing success rates. From single-gene cloning to operon-level assembly, empower your Design–Build–Test cycle with a robust solution trusted by molecular biologists and synthetic biologists alike.

• High success rate

Tested in nearly 30 different assembly systems, ClonExpress Ultra (Vazyme #C117) consistently delivers a positive clone rate above 95%—a clear testament to its exceptional accuracy and reliability in seamless DNA assembly.

• High Clone Yield Across Complex Assemblies

Vazyme ClonExpress Ultra (#C117) supports seamless assembly of 1 to 11 DNA fragments, offering exceptional flexibility for various cloning workflows.

In comparative tests across nearly 30 different systems, ClonExpress Ultra consistently generated more colonies than equivalent kits from Supplier A, Supplier B, and Supplier C—demonstrating superior cloning efficiency and robust performance under diverse conditions.

• Broad Compatibility with Low-Input Tolerance

Even with just one-tenth of the standard DNA input, Vazyme ClonExpress Ultra (#C117) consistently outperformed comparable kits from Supplier A, Supplier B, and Supplier C—yielding significantly higher colony counts and positive clone rates. This exceptional sensitivity and robustness make it an ideal choice for low-input cloning workflows, where performance under limiting conditions is critical.

*Fragment input amount: 0.006 pmol  (1/10 of the standard input amount)

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Efficient clone screening is critical to accelerating molecular cloning workflows. 2× Rapid Taq Master Mix(Vazyme #P223) is optimized for fast, reliable identification of positive colonies—no plasmid purification required. Designed to amplify directly from bacterial or fungal colonies, it delivers robust results across hosts like E. coli and yeast, while maintaining high specificity and broad compatibility. With simplified protocols and minimal hands-on time, Vazyme enables you to go from colony to confirmed clone with confidence and efficiency.

• Ultra-Fast Amplification in Just 30 Minutes

When amplifying a 2 kb DNA fragment under each manufacturer's recommended cycling conditions, 2× Rapid Taq Master Mix(Vazyme #P223) completed the reaction in just 30 minutes—significantly faster than comparable products from Supplier A and Supplier B. This ultra-fast performance highlights the efficiency of P223 for time-sensitive molecular cloning applications.

• High Success Rate Across GC Content and Sample Types

1. Broad GC Compatibility

2× Rapid Taq Master Mix(Vazyme #P223) and five commercially available Taq DNA polymerases (Supplier A–E) were evaluated for their performance on templates with varying GC content.

The results showed that P223 successfully amplified targets with GC contents ranging from 24% to 79%, demonstrating exceptional compatibility across both AT-rich and GC-rich regions. Moreover, P223 consistently outperformed all comparator enzymes in both amplification success rate and specificity, confirming its robust performance under challenging sequence conditions.

2. Broad Template Compatibility

Using the same group of polymerases (P223 and Supplier A–E), different template types were tested under each manufacturer's recommended extension conditions.

P223 showed strong compatibility with a wide variety of crude sample types—including animal and plant lysates, bacterial colonies, and liquid cultures. In all tested conditions, P223 delivered higher success rates, better specificity, and greater yield than the competitor products, underscoring its versatility and reliability for diverse molecular biology applications.

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