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  • Name: HiScript II 1st Strand cDNA Synthesis Kit
  • Number: R211
  • 币种: 美元
    • SIZE: 50 rxn 100 rxn

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HiScript II 1st Strand cDNA Synthesis Kit

For high cost-effective first-strand cDNA synthesis Kit

• Based on high-effective HiScript II Reverse Transcriptase: with high thermal stability ensuring the complex RNA secondary structure be denatured to obtain longer cDNA.

• A wide range of template input amount of total RNA (from 1 pg to 5 μg). Amplify cDNA fragments as long as 20 kb.

• Anchored Oligo(dT)23VN: further improves the specificity, efficiency and sensitivity of the first-strand cDNA synthesis.

• With different primer selection strategy, the first-strand cDNA products can be widely used for many applications, including cloning, hybridization, PCR amplification, and Real Time PCR.


Fig. 1. cDNA fragments as long as 20 kb was successfully obtained from RNA templates using R211.

Reverse transcription was performed using 1 μg total RNA of HeLa cells or total RNA (for Nebulin) of skeletal muscle cells as templates and Oligo(dT)23VN as primers. PCR amplification was performed using 1 μl of cDNA (as template) and Vazyme LAmp DNA Polymerase.

M: DL 15000 Marker.

Fig. 2. R211 has a wide and sensitive linear reverse transcription range for qPCR

Reverse transcription was perform using 1 μg-1 pg total RNA of Hela cells as template and random hexamers/Oligo(dT)23VN as mixed primers. QPCR amplification of amplify ACTB gene and BR3 gene was performed using 1 μl of cDNA (as template) and AceQ qPCR SYBR® Green Master Mix, respectively.

Fig. 3. R211 has higher reverse transcription efficiency for complex RNA templates.

Using 1 μg of total RNA of Hela cells as template, reverse transcription was performed using Vazyme #R211 and a similar product of T brand, respectively. The reaction time is 5 min. Using 1 μl of cDNA as template, the high-GC regions of FIB (6100 bp) and Pole (7117bp) were amplified using AceQ qPCR SYBR® Green Master Mix, respectively.

The HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. The half-life of HiScript II at 50°C is > 240 min. Even at 55°C, the HiScript II can stay stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structures. In addition, the HiScript II has a improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (as long as 20 kb).

The HiScript II 1st Strand cDNA Synthesis Kit contains all the components necessary for the 1st strand cDNA synthesis. The products are suitable for PCR and qPCR. The 2× RT Mix contains an optimized buffer and dNTPs. The HiScript II Enzyme Mix contains the HiScript II Reverse Transcriptase and the RNase inhibitor. The Oligo-(dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.

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