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  • Name: Taq Plus DNA Polymerase
  • Number: P201
  • 币种: 美元
    • SIZE: 250 U 1000 U 3000 U 250 U (with dNTP) 1000 U (with dNTP) 3000 U (with dNTP)

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Taq Plus DNA Polymerase

Taq Plus DNA polymerase is a mixture of Taq DNA polymerase and an enzyme containing 3’→5’ exonuclease activity. The fidelity of Taq Plus is 6 times greater than that of Taq DNA Polymerase. Compared with Taq DNA Polymerase, Taq Plus DNA polymerase has stronger amplification performance, higher sensitivity and yield, and is more tolerant of impurities within 5 kb amplifying range. The obtained PCR products are compatible with ClonExpress II One Step Cloning Kit series (Vazyme, #C112, #C113). The PCR products contain A at the 3’-end and can be directly cloned into T-Vectors.

Components 

P201-01 250 U

 P201-d1  250 U

P201-02/d2   1,000 U

  P201-03/d  3,000 U

10 × Taq Plus Buffer (Mg2+ plus)

1 ml

1 ml

4 ml

 

dNTP Mix (10 mM each)

------

200 µl

± 800 µl

 

Taq Plus DNA Polymerase (5 U/μl)

50 µl

50 µl

200 µl

 P201-02/d2 × 3

 

Store at -20℃.

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