LOGO
Search
Submit
Cancel
这是描述信息
/
/
/
/
/
HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper)

NGS Library Prep kits

Molecular Research

Drug Discovery

1/1
Number of views:
6435

HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper)

HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance. The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR   Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.; gDNA wiper Mix can quickly and completely remove genome contamination   Store at -20℃
Retail price
$
900.0
Market price
$
0.0
Number of views:
6435
Product serial number
R212-01/02
Quantity
-
+
Stock:
3996
暂时无货
1
Detail
FAQ
References
CoA Report
Download

HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance.
The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR

 

Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA;
A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more;
Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis;
Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.;
gDNA wiper Mix can quickly and completely remove genome contamination

 

Store at -20℃

Keyword:
R212-01/02
We could not find any corresponding parameters, please add them to the properties table

 

 

 
  • R212 HiScript II 1st Strand cDNA Synthesis Kit(+gDNA wiper).pdf

    Size:
    2021-03-16 11:16:22
Previous page
1

The products on this website may not be sold in all countries or regions. Please contact Vazyme or your local distributor for further information.

这是描述信息

Add:Nanjing State Economy & Technology Development Zone, Red Maple Technology Industrial Park, building C 1-2 Tel:400-600-9335
Copyright 2015 Vazyme Biotech Co.,Ltd All rights reserved 
苏ICP备12014779号 Power by:www.300.cn nanjing.300.cn