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NGS Library Prep kits

Molecular Research

Drug Discovery

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HiScript Reverse Transcriptase

HiScript® Reverse Transcriptase is a new reverse transcriptase based on M-MLV (RNase H-) Reverse Transcriptase through multiple mutations. HiScript® Reverse Transcriptase has the highest activity at 50°C and can withstand reaction temperatures up to 55°C, and can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning.    Tolerate a reaction temperature of 50℃, suitable for RNA templates with secondary structure; The template has strong affinity, and more full-length cDNA can be obtained; Highly sensitive, can detect as little as 10 pg of total RNA   Store at -20℃
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Product serial number
R101-01/02
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HiScript® Reverse Transcriptase is a new reverse transcriptase based on M-MLV (RNase H-) Reverse Transcriptase through multiple mutations. HiScript® Reverse
Transcriptase has the highest activity at 50°C and can withstand reaction temperatures up to 55°C, and can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning. 

 

Tolerate a reaction temperature of 50℃, suitable for RNA templates with secondary structure;
The template has strong affinity, and more full-length cDNA can be obtained;
Highly sensitive, can detect as little as 10 pg of total RNA

 

Store at -20℃

Keyword:
R101-01/02
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