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NGS Library Prep kits

Molecular Research

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M-MLV (H-) Reverse Transcriptase

Wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, this product retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction, etc. It also has strand displacement activity and can be used in experiments such as 5'RACE.   Point mutations eliminate RNase H activity to obtain long cDNA products; Stable and reliable reverse transcription performance for RNA templates above 100 ng; Synthetic fragment ≤5 kb; Can be used for experiments such as 5’-RACE reaction and cDNA library construction   Store at -20℃
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Product serial number
R021-01
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Wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, this product retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction, etc. It also has strand displacement activity and can be used in experiments such as 5'RACE.

 

Point mutations eliminate RNase H activity to obtain long cDNA products;
Stable and reliable reverse transcription performance for RNA templates above 100 ng;
Synthetic fragment ≤5 kb;
Can be used for experiments such as 5’-RACE reaction and cDNA library construction
 

Store at -20℃

Keyword:
R021-01
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Liu X ,Wei W,Li X , et al. (2017). BMI1 and MEL18 Promote Colitis-Associated Cancer in Mice via REG3B and STAT3.Gastroenterology.IF:18.392

Ju J, Chen A, Deng Y, et al. (2017).NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression.[J]. Nature Communications, 2017, 8(1):928.IF:12.124

Ding W, Liu WQ, Jia Y, et al. (2016). Biosynthetic investigation of phomopsins reveals a widespread pathway for ribosomal natural products in Ascomycetes.PNAS, 113(13):3521-3526. IF:9.674

Wang H, Liang L, Dong Q,et al.(2018)Long noncoding RNA miR503HG, a prognostic indicator, inhibits tumor metastasis by regulating the HNRNPA2B1/NF-κB pathway in hepatocellular carcinoma. Theranostics.IF:8.172

Zhang B, Wang M, Gong A, et al. (2015). HucMSC-exosome mediated -Wnt4 signaling is required for cutaneous wound healing. Stem Cells, 33(7):2158-2168.IF:7.133

Zhang Y, Du J, Zheng J, et al. (2015). EGF-reduced Wnt5a transcription induces epithelial-mesenchymal transition via Arf6-ERK signaling in gastric cancercells. Oncotarget. IF:6.627

Balamurugan S, Wang X, Wang H L, et al. (2017). Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylumtricornutum[J]. Biotechnology for Biofuels, 2017, 10(1):97.IF:6.444

Wang Y, Huang Y, Xu Y, et al. (2017).A Dual AMPK/Nrf2 Activator Reduces Brain Inflammation after Stroke by Enhancing Microglia M2 Polarization[J]. Antioxidants & Redox Signaling, 2017.IF:6.337

Gu J, Wang D, Zhang J, et al. (2016). GFRalpha2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer. Cancer Lett, 380(2), 434-441. IF:5.992

Wang Y, Ruan W, Mi J,et al. (2017).Balasubramide derivative 3C modulates microglia activation via CaMKKβ-dependent AMPK/PGC-1α pathway in neuroinflammatory conditions[J]. Brain Behavior & Immunity, 2017.IF:5.964

Si Z, Liu H, Zhu J, et al.2018Mutation ofSELF-PRUNINGhomologs in cotton promotes short-branching plant architecture:[J]. Journal of Experimental Botany, 2018, 69(10):2543-2553.IF5.83

Zhu J, Chen J, Gao F, et al. 2017Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis-next generation sequencing and virus-induced gene silencing strategies.[J]. Journal of Experimental Botany, 2017, 68(15):4125.IF5.83

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