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NGS Library Prep kits

Molecular Research

Drug Discovery

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2640

XL10 Competent E.coli Strain

This product is prepared from chemically competent cells for cloning using a modified Inoue method, which enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacterial cells. The transformation efficiency exceeds 108 cfu/μg, which is widely used in plasmid transformation, gene cloning, blue-white spot screening and other purposes. Improved Escherichia coli enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacteria cells. Genotype Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lacTetr Hte [F′ proAB lacI qZΔM15 Tn10(Tet r) Amy Camr]   XL10 strain, resistant to tetracycline (Tetr) and chloramphenicol (Camr), a new cloned strain; Suitable for efficient conversion of PCR products, cDNA and other sources of unmethylated DNA; EndA1, RecA1 mutations ensure the stability of foreign DNA in the host bacteria and facilitate the extraction of high-quality plasmid DNA; Hte genotype, enhances the transformation efficiency of large fragments of DNA, and greatly increases the growth rate of host bacterial cells; No foreign plasmid DNA residue, transformation efficiency ≥108 cfu/μg pUC19   Store at -80°C (do not store in -20°C or liquid nitrogen!)
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Number of views:
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Product serial number
C503-02/03
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This product is prepared from chemically competent cells for cloning using a modified Inoue method, which enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacterial cells. The transformation efficiency exceeds 108 cfu/μg, which is widely used in plasmid transformation, gene cloning, blue-white spot screening and other purposes. Improved Escherichia coli enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacteria cells. Genotype Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lacTetr Hte [F′ proAB lacI qZΔM15 Tn10(Tet r) Amy Camr]

 

XL10 strain, resistant to tetracycline (Tetr) and chloramphenicol (Camr), a new cloned strain;
Suitable for efficient conversion of PCR products, cDNA and other sources of unmethylated DNA;
EndA1, RecA1 mutations ensure the stability of foreign DNA in the host bacteria and facilitate the extraction of high-quality plasmid DNA;
Hte genotype, enhances the transformation efficiency of large fragments of DNA, and greatly increases the growth rate of host bacterial cells;
No foreign plasmid DNA residue, transformation efficiency ≥108 cfu/μg pUC19

 

Store at -80°C (do not store in -20°C or liquid nitrogen!)

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C503-02/03
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