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NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
6212

DH5α 化学感受态细胞

The DH5α strain is the most commonly used competent cell in the laboratory. The deletion of endonuclease (endA) improves the yield and quality of plasmid DNA; the defective recombinase (recA) reduces the probability of recombination of inserts and ensures the stability of inserted DNA; the existence of lacZ ΔM15 makes DH5α available Screening for blue and white spots. The company's DH5α competent cells are produced through a special process, and the transformation efficiency of pUC19 plasmid detection is ≥108 cfu/μg DNA.   Suitable for efficient conversion of PCR products, cDNA and other sources of unmethylated DNA; EndA1, RecA1 mutations ensure the stability of foreign DNA in the host bacteria and facilitate the extraction of high-quality plasmid DNA; Can be used for blue and white screening of recombinant plasmids; Transformation efficiency≥108cfu/μg pUC19 DNA   Store below -70°C (do not store in -20°C or liquid nitrogen!)
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Product serial number
C502-02/03
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The DH5α strain is the most commonly used competent cell in the laboratory. The deletion of endonuclease (endA) improves the yield and quality of plasmid DNA; the defective recombinase (recA) reduces the probability of recombination of inserts and ensures the stability of inserted DNA; the existence of lacZ ΔM15 makes DH5α available Screening for blue and white spots. The company's DH5α competent cells are produced through a special process, and the transformation efficiency of pUC19 plasmid detection is ≥108 cfu/μg DNA.

 

Suitable for efficient conversion of PCR products, cDNA and other sources of unmethylated DNA;
EndA1, RecA1 mutations ensure the stability of foreign DNA in the host bacteria and facilitate the extraction of high-quality plasmid DNA;
Can be used for blue and white screening of recombinant plasmids;
Transformation efficiency≥108cfu/μg pUC19 DNA

 

Store below -70°C (do not store in -20°C or liquid nitrogen!)

Keyword:
C502-02/03
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Wang M, Zhao Q, Zhang Q, et al. (2016). Differences in PLP-Dependent Cysteinyl Processing Lead to Diverse S-Functionalization of Lincosamide Antibiotics. J Am ChemSoc, 138(20), 6348-6351.  IF: 13.038
Yuan H, Zhang J, Cai Y, et al. GyrI-like proteins catalyze cyclopropanoid hydrolysis to confer cellular protection[J]. Nature Communications, 2017, 8(1).IF:12.124
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