T4 DNA Ligase can catalyze the formation of phosphodiester bonds between the 5'phosphate end and 3'hydroxyl end of the adjacent nucleic acid at the blunt or sticky end of dsDNA. It can also catalyze the connection between RNA and ssDNA or RNA strands in the double strand, but it cannot catalyze the entire single Chain nucleotide connection. Suitable for nucleic acid operations such as labeling RNA 3'-end, circularizing RNA and DNA oligonucleotides and cloning cDNA.   Purity is greater than 99%, no exogenous nuclease activity remains; The classic buffer composition allows the ligation reaction to proceed efficiently   Store at- 20°C