
NGS Library Prep kits
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DNA-Seq
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RNA-Seq
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Single Cell-Seq
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Epigenetics
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Beads
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library Quantification
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cfDNA
Molecular Research
Drug Discovery
Detergent Compatible Bradford Protein Quantification Kit
Detergent Compatible Bradford Protein Quantification Kit
通用型蛋白定量试剂
检测蛋白的灵敏度高,操作简便
线性关系好
对去垢剂有很强的耐受性,可兼容很高浓度的去垢剂
稳定性好,BSA标准蛋白更纯
图1. 使用BSA标准蛋白绘制标准曲线
Bradford 法( 考马斯亮蓝法),是目前灵敏度最高的蛋白质浓度测定方法之一。当Bradford 染色液( 考马斯亮蓝G250) 和蛋白在酸性条件下结合时,溶液颜色由棕黑色转为蓝色,最大吸光值波长由456 nm 转为595 nm,吸光值与蛋白质的含量在一定浓度范围内有较好的线性关系。通过测定吸光值大小并对照标准蛋白的吸光值,推算出蛋白浓度,实现了蛋白浓度测定的快速,稳定和高灵敏度。
本试剂通过研发升级,对蛋白提取中常见的去垢剂有很高的耐受性。本试剂盒和常规的Bradford 蛋白浓度测定试剂盒相比,可以兼容一系列常见的去垢剂,克服了Bradford 法对去垢剂敏感的缺点;和BCA法相比,能够兼容高浓度的还原剂,并且检测速度极快,在检测含去垢剂样品蛋白浓度时,更加快捷。
组分 |
E211-01 (500 rxn) |
BSA Standard (20 mg/ml) |
2 × 1 ml |
Bradford Reagent |
150 ml |
Bradford Reagent 2℃ ~8℃保存,BSA Standard -30℃ ~-15℃保存
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