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Hyperactive In-Situ ChIP Library Prep Kit for Illumina(pA-Tn5)

NGS Library Prep kits

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Hyperactive In-Situ ChIP Library Prep Kit for Illumina(pA-Tn5)

HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a special kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interaction. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Simple operation: using enzyme digestion instead of ultrasonic disruption, it only takes 1 day to realize the transformation from cells to next-generation sequencing libraries High activity: DNA fragmentation activity is extremely high Low starting cell volume: compatible with as few as 50 cells High purity: high protein purity, low nucleic acid residue     BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature. BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week; 10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months; Store the remaining components at -30 ~ -15°C.  
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1124.0
Number of views:
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Product serial number
TD902-01/02
Category
CUT&Tag
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HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a special kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interaction. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.

 

Simple operation: using enzyme digestion instead of ultrasonic disruption, it only takes 1 day to realize the transformation from cells to next-generation sequencing libraries
High activity: DNA fragmentation activity is extremely high
Low starting cell volume: compatible with as few as 50 cells
High purity: high protein purity, low nucleic acid residue
 

 

BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature.
BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week;
10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months;
Store the remaining components at -30 ~ -15°C.
 

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  • TD901 TD902 V9.1 Hyperactive In-Situ ChIP Library Prep Kit for Illumina.pdf

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