Amplification Mix2 is a new type of high-fidelity PCR amplification master mix, suitable for PCR amplification of high-throughput sequencing libraries. The master mix is ​​based on VAHTSTM Super-Fidelity DNA Polymerase, which is a new generation of high-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and its amplification mismatch rate is 1/52 of Taq DNA Polymerase is 1/6 of Pfu DNA Polymerase. With a carefully optimized reaction buffer, Amplification Mix2 can achieve low-preference, high-efficiency, and high-stability amplification of high-throughput sequencing libraries. This product contains all the components required for PCR amplification, and only needs to add template primers to perform the reaction, which is convenient to use; it also contains a special protective agent to maintain stable activity after long-term storage or repeated freezing and thawing. The amplified product is blunt-ended and can be directly used for blunt-ended cloning.   Contains 207 primer pairs that can be used to amplify hot mutation regions of human tumor-related genes; The products cover a total of 2800 common mutation sites of 50 proto-oncogenes and tumor suppressor genes   Store at -20°C, valid for one year