VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina v2 is a special kit for the construction of strand-specific transcriptome libraries developed for the Illumina high-throughput sequencing platform. This kit is suitable for starting template amount of 0.05-4 μg completeness A good chain-specific transcriptome library construction of total RNA of eukaryotes such as animals, plants, and fungi. Unlike conventional transcriptional library construction, the substrate is incorporated into dUTP during the synthesis of the second strand, and is enriched by PCR Use UDG enzyme for the U-labeled second-strand template before the library Digestion ensures that the final sequencing information comes from the first-strand cDNA, thereby preserving the strand directionality of the mRNA. Later data analysis can determine whether the transcript comes from the sense or antisense strand of DNA. This kit is an upgraded product of VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina. This version greatly simplifies the operation process, improves the library conversion efficiency, is compatible with a lower initial input, and uses magnetic bead sorting. A library of a specific length can be quickly obtained to meet the individual needs of different experiments. All enzymes and buffers included in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.    Box1: Store at 2 ~ 8℃; Box2: Store at -30 ~ -15℃.