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NGS Library Prep kits

Molecular Research

Drug Discovery

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DNA polymerase I Klenow fragment

DNA polymerase I, the large fragment (Klenow fragment) is the protease hydrolysate of E. coli DNA polymerase I, with 5´→3´ DNA polymerase activity and 3´→5´ exonuclease activity, but 5 is missing ´→3´ Exonuclease activity. The Klenow fragment retains the high fidelity of the holoenzyme without degrading the 5´ end of DNA.   Simple and rapid isothermal reaction speeds up the preparation of plasmid samples for sequencing; High fidelity, strong chain switching activity, suitable for whole genome amplification    Store at -20℃
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N104-01
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DNA polymerase I, the large fragment (Klenow fragment) is the protease hydrolysate of E. coli DNA polymerase I, with 5´→3´ DNA polymerase activity and 3´→5´ exonuclease activity, but 5 is missing ´→3´ Exonuclease activity. The Klenow fragment retains the high fidelity of the holoenzyme without degrading the 5´ end of DNA.

 

Simple and rapid isothermal reaction speeds up the preparation of plasmid samples for sequencing;
High fidelity, strong chain switching activity, suitable for whole genome amplification

 

 Store at -20℃

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polymerase
dna
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klenow
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We could not find any corresponding parameters, please add them to the properties table

Q1:N104与N101的区别?

A1:N104具有5´→3´ DNA 聚合酶活性和3´→5´核酸外切酶活性,但缺失了5´→3´核酸外切酶活性。它既具有全酶的高保真性,又不会降解 DNA 5´末端。与N101相比,N104除了能够切除片段化DNA的3′ 突出端,还能补平5′ 突出端,形成平末端结构。

 

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