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NGS Library Prep kits

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VAHTS mRNA-seq V2 Library Prep Kit for Illumina

VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for animals and plants with an initial template amount of 0.1-4 μg And fungi and other eukaryotic total RNA. The total RNA sample goes through the steps of mRNA isolation, fragmentation, double-stranded cDNA synthesis, end repair, A-tailing, adaptor ligation, ligation product purification, fragment size sorting, library amplification, etc. Finally, a sequencing library suitable for the Illumina® platform is obtained. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. The kit includes three NR1, NR2 and NR3 The independent packaging contains all the enzymes and buffers required for the library construction process. All components have undergone strict quality control and functional verification to ensure the stability and repeatability of the library construction to the greatest extent. Adapters and DNA purification magnetic beads need to be prepared separately. Adapters can be selected from VAHTSTM RNA Adapters (Vazyme #N803, Vazyme #N804 or Vazyme #N809, Vazyme #N810, Vazyme #N811, Vazyme #N812). For details on adapter selection, please refer to Common Problems and Solutions 4 (Page16); DNA purification magnetic Beads can choose VAHTSTM DNA Clean Beads (Vazyme #N411) or Agencourt® AMPure® XP reagent (Beckman #A63880, #A63881, #A63882).    The chain directionality of the mRNA is retained, and the gene structure and gene expression information can be obtained more accurately; It has a high success rate for complex plant templates; The library construction process is the same as Illumina TruSeq Stranded mRNA Sample Prep Kit; Optimized large fragment library construction program; The sequencing data is highly consistent with Illumina reagents   NR 1, 2-8°C storage; NR 2, stored at -20°C; NR 3, stored at -20°C
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VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for animals and plants with an initial template amount of 0.1-4 μg And fungi and other eukaryotic total RNA. The total RNA sample goes through the steps of mRNA isolation, fragmentation, double-stranded cDNA synthesis, end repair, A-tailing, adaptor ligation, ligation product purification, fragment size sorting, library amplification, etc. Finally, a sequencing library suitable for the Illumina® platform is obtained. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. The kit includes three NR1, NR2 and NR3 The independent packaging contains all the enzymes and buffers required for the library construction process. All components have undergone strict quality control and functional verification to ensure the stability and repeatability of the library construction to the greatest extent.
Adapters and DNA purification magnetic beads need to be prepared separately. Adapters can be selected from VAHTSTM RNA Adapters (Vazyme #N803, Vazyme #N804 or Vazyme #N809, Vazyme #N810, Vazyme #N811, Vazyme #N812). For details on adapter selection, please refer to Common Problems and Solutions 4 (Page16); DNA purification magnetic Beads can choose VAHTSTM DNA Clean Beads (Vazyme #N411) or Agencourt® AMPure® XP reagent (Beckman #A63880, #A63881,
#A63882). 

 

The chain directionality of the mRNA is retained, and the gene structure and gene expression information can be obtained more accurately;
It has a high success rate for complex plant templates;
The library construction process is the same as Illumina TruSeq Stranded mRNA Sample Prep Kit;
Optimized large fragment library construction program;
The sequencing data is highly consistent with Illumina reagents

 

NR 1, 2-8°C storage;
NR 2, stored at -20°C;
NR 3, stored at -20°C

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