This kit is suitable for extracting 1-5 ml of bacteria cultured overnight. The optimized SDS-alkaline lysis method is used to lyse cells. The extraction of multiple samples can be completed in 30 minutes. The extraction process does not require phenol-chloroform extraction or ethanol precipitation. Under the conditions of high salt and low pH, the plasmid DNA in the solution is efficiently and specifically combined, and the protein, genome, RNA and other impurities are removed to the maximum extent. Finally, the pure plasmid DNA is removed from the silicon in the low salt and high pH elution buffer. Elution on the matrix membrane, each adsorption column can adsorb up to 35 μg of plasmid DNA. The purified plasmid DNA can be directly used in biological experiments such as restriction digestion, PCR, sequencing, ligation, transformation, and transfection of some conventional passage cells.   Simple and fast; High purity; High Yield   Please store RNase A at -20℃, and store other components at room temperature (15-25℃)