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NGS Library Prep kits

Molecular Research

Drug Discovery

High-Fidelity PCR

56.00
Article number:
P525-01/02/03
Phanta® Max has added unique elongation factors, specificity promoting factors and plateau uninhibiting factors, which greatly improves the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can effectively amplify fragments up to 20 kb; using cDNA templates, Phanta® Max It can effectively amplify fragments up to 10 kb. Its amplification mismatch rate is 1/53 of that of ordinary Taq polymerase and 1/6 of that of Pfu polymerase. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max contains two monoclonal antibodies that can inhibit its 5’ → 3’ polymerase activity and 3’ → 5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the system allows the 2 × Master mix to maintain stable activity after repeated freezing and thawing; the system contains an electrophoresis indicator, which can be directly spotted for electrophoresis after the PCR reaction is completed. The enzyme has 5'→ 3'polymerase activity and 3'→ 5'exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress® and topological cloning kits (C112/C113/C115/C601).   Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb. Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples Simple operation: Dye premix, minimize experimental steps    -Store at 20°C  
56.00
Article number:
P525-01/02/03
Number of views:
4911
Keywords:
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56.00
Article number:
P515-01/02/03
Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specificity promoting factors, and plateau uninhibitory factors, which have greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Increased. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can Effectively amplify fragments up to 10 kb. The mismatch rate is 1/53 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added with two monoclonal antibodies that can inhibit its 5’→3’ polymerase activity and 3’→5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added in the system allows 2 × Master mix to maintain stable activity after repeated freezing and thawing. The amplified product is blunt-ended and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).   Super fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are less than 5 mismatches; Amplify faster: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: simple templates such as plasmids and lambda DNA can be amplified up to 40 kb, cDNA can be amplified up to 10 kb, and genome can be amplified up to 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.   Store at -20°C
56.00
Article number:
P515-01/02/03
Number of views:
15037
Keywords:
phanta
and
of
to
can
the
dna
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is
9999.00
Article number:
P511-01/02/03
Phanta® Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. With the genetic engineering modification of Pfu DNA Polymerase, the processivity of Phanta® Super-Fidelity DNA Polymerase has been greatly improved, even for very complex templates, the reaction can be completed accurately and quickly. The mismatch rate is the normal Taq 1/52 of the enzyme is 1/6 of the Pfu enzyme; and the amplification speed can reach 15 seconds/kb. The high fidelity and excellent amplification efficiency make Phanta® Super-Fidelity DNA Polymerase the first choice for high-fidelity PCR. This product contains Phanta® Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It can be amplified by adding primers and templates, reducing pipetting operations and extracting High throughput and reproducibility of results. The protective agent added to the system allows Master Mix to maintain stable activity after repeated freezing and thawing. The amplified product is blunt-ended, suitable for ClonExpress® Rapid cloning kit (C112/C113).    The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various templates; Ready-to-use premix, minimizing experimental steps   Store at -20°C
9999.00
Article number:
P511-01/02/03
Number of views:
6524
Keywords:
87.00
Article number:
P505-d1/d2/d3
Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specific promotion factors and plateau release inhibitors. It has greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as lambda DNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using genomic DNA, etc. For complex templates, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can effectively amplify fragments up to 10 kb. Its mismatch rate is 1/53 of that of ordinary Taq enzyme, and it is Pfu enzyme 1/6. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added to inhibit at room temperature Its two monoclonal antibodies with 5'→3' polymerase activity and 3'→5' exonuclease activity can perform highly specific hot-start PCR. The amplified products are blunt-ended and are suitable for ClonExpress® fast Rapid cloning kit (C112/C113/C114).    Super fidelity: the fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. Every 300,000 bases amplified, with less than 5 mismatches; Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of cDNA can reach 10 kb, and the effective amplification length of genome can reach 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.   Store at -20℃, avoid repeated freezing and thawing
87.00
Article number:
P505-d1/d2/d3
Number of views:
21569
Keywords:
9999.00
Article number:
P504-d1/d3
Phanta Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. With the genetic engineering of Pfu DNA Polymerase, the processivity of Phanta Super-Fidelity DNA Polymerase has been greatly improved. Even for very complex templates, the amplification reaction can be completed accurately and quickly. Phanta EVO HS Super-Fidelity DNA Polymerase is an upgraded version of Phanta HS Super-Fidelity DNA Polymerase. Compared with the previous generation product, Phanta EVO HS has added a unique extension factor and deeply optimized the reaction system to further improve its amplification stability, fidelity and ability to amplify long fragments. Using simple templates such as lambda DNA and plasmids, Phanta EVO HS can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta EVO HS can also effectively amplify fragments up to 20 kb. Its amplification mismatch rate is 1/100 of that of ordinary Taq polymerase and 1/12 of Pfu polymerase; and the amplification speed can reach 15 seconds/kb. In addition, Phanta EVO HS has good resistance to PCR inhibitors and can be used for bacteria, fungi, Direct PCR of plant tissue, animal tissue or whole blood samples. Strict fidelity performance and excellent amplification efficiency make Phanta EVO HS the first choice for high-fidelity PCR. Phanta EVO HS has added two monoclonal antibodies that can inhibit its 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling highly specific Hot Start PCR . The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplified product is blunt-ended, which is suitable for ClonExpress rapid cloning kit (C112/C113). 5 × EVO Buffer has good adaptability to PCR amplification of simple or complex templates, short fragments or long fragments. It already contains 10 mM Mg2+, you can use the MgCl2 or PCR Enhancer provided with the enzyme to optimize the reaction system.    Super fidelity: the fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. Every 300,000 bases amplified, with less than 5 mismatches; Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of cDNA can reach 10 kb, and the effective amplification length of genome can reach 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.   Store at -20°C
9999.00
Article number:
P504-d1/d3
Number of views:
2562
Keywords:
600.00
Article number:
P503-d1/d3
Phanta Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. With the genetic engineering of Pfu DNA Polymerase, the processivity of Phanta Super-Fidelity DNA Polymerase has been greatly improved, even for very complex templates, the amplification reaction can be completed accurately and quickly. Phanta EVO Super-Fidelity DNA Polymerase It is an upgraded version of Phanta Super-Fidelity DNA Polymerase. Compared with the previous generation product, Phanta EVO has added a unique extension factor and deeply optimized the reaction system to make its amplification stability, fidelity and long-term The amplification ability of fragments has been further improved. Using simple templates such as lambda DNA and plasmids, Phanta EVO can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta EVO can also effectively amplify fragments up to 20 kb. Fragment. Its amplification mismatch rate is 1/100 of that of ordinary Taq polymerase and 1/12 of Pfu polymerase; and the amplification speed can reach 15 seconds/kb. In addition, Phanta®EVO is good for PCR inhibitors Resistance ability, can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Rigorous fidelity performance and excellent amplification efficiency make Phanta EVO a high-fidelity PCR Enzymes are the first choice. The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress rapid cloning kit (C112/C113). 5 × EVO Buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. It already contains 10 mM Mg2+, you can use the MgCl2 or PCR Enhancer provided with the enzyme to optimize the reaction system.    Effectively amplify 20 kb genome fragments and 40 kb plasmid fragments; The fidelity is 100 times that of Taq DNA Polymerase and 12 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various types of templates, which is convenient for obtaining ideal amplification results quickly   Store at -20°C
600.00
Article number:
P503-d1/d3
Number of views:
3608
Keywords:
9999.00
Article number:
P502-d1/d3
Phanta® Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. After genetic engineering of Pfu DNA Polymerase, the processivity of Phanta® Super-Fidelity DNA Polymerase has been greatly improved, even for very complex templates, it can complete the reaction accurately and quickly. The mismatch rate is 1/52 that of ordinary Taq enzyme and 1/6 that of Pfu enzyme; and the amplification speed can reach 15 seconds/kb. High fidelity and excellent amplification efficiency make Phanta® Super-Fidelity DNA Polymerase the enzyme of choice for high-fidelity PCR. On the original basis, Phanta® HS Super-Fidelity DNA Polymerase is added with two monoclonal antibodies that can inhibit 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which can perform highly specific Thermal start (Hot Start) PCR. Phanta® HS Super-Fidelity DNA Polymerase has 5’→3’ polymerase activity and 3’→5’ exonuclease activity. The amplified product is blunt-ended, suitable for ClonExpress® rapid cloning kit (C112/C113). 5 × Phanta SF reaction buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration is 2 mM). The reaction system can be optimized using the DMSO or MgSO4 provided with the enzyme. The attached PCR Enhancer can help the amplification of high GC content fragments.   Added monoclonal antibody, can perform highly specific hot-start PCR; The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various templates; Provide PCR Enhancer to help amplify templates with high GC content   Store at -20°C
9999.00
Article number:
P502-d1/d3
Number of views:
3519
Keywords:
9999.00
Article number:
P501-d1/d2/d3
Phanta®Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. After genetic engineering modification of Pfu DNA Polymerase, the processivity of Phanta®Super-Fidelity DNA Polymerase has been greatly improved. Even for very complex templates, the reaction can be completed accurately and quickly. Its mismatch rate is 1/52 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme, and its amplification speed can reach 15 seconds/kb. 5 × Phanta SF reaction buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration is 2 mM). The reaction system can be optimized using the DMSO or MgSO4 provided with the enzyme. The attached PCR Enhancer can help the amplification of high GC content fragments.   The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; Wide template adaptability; The optimized buffer system is widely applicable to various types of templates, which is convenient for obtaining ideal amplification results quickly   Store at -20°C
9999.00
Article number:
P501-d1/d2/d3
Number of views:
7425
Keywords:

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这是描述信息

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