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NGS Library Prep kits

Molecular Research

Drug Discovery

Modules for DNA Library Prep

48.00
Article number:
N106-01
48.00
Article number:
N106-01
Number of views:
1000
Keywords:
71.00
Article number:
P507-01/02
Phanta® Uc Super-Fidelity DNA Polymerase is a new generation of high-fidelity DNA polymerase modified from Pfu DNA Polymerase, with extremely high amplification efficiency Rate and wide template adaptability. Compared with Pfu DNA Polymerase, Phanta® Uc Super-Fidelity DNA Polymerase has greatly improved its mobility. Even very complex templates can complete PCR reactions quickly and accurately. Its mismatch rate is 1/52 of Taq DNA Polymerase and 1/6 of Pfu DNA Polymerase, and it completely overcomes the inability of conventional high-fidelity enzymes to use dUTP as raw material for polymerization, the inability to use amplification primers containing dUTP, and the inability to use DNA containing dUTP is used as an amplification template and many other disadvantages. With a carefully optimized reaction buffer for library amplification, Phanta® Uc Super-Fidelity DNA Polymerase for Library Amplification can achieve high-throughput measurement The low preference, high efficiency and high stability amplification of sequence library. The amplified product is blunt-ended and can be directly used for blunt-ended cloning.    Gap filling (no chain displacement activity); Cut off the 3'protruding end or fill in the 5'protruding end to form a flat end   All components stored at -20°C
71.00
Article number:
P507-01/02
Number of views:
2556
Keywords:
amplification
dna
polymerase
and
the
of
is
to
for
48.00
Article number:
N106-01/02
Phi29 MAX DNA Polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29. Phi29 MAX DNAPolymerase has strong strand displacement activity, which can realize the melting and replication of complex DNA structures, and perform isothermal DNA polymerization reactions in vitro that do not rely on thermal cycling. Phi29 MAX DNA Polymerase has strong chain affinity. A single polymerization reaction can achieve continuous polymerization extension up to 100 kb. In addition, Phi29 MAX DNA Polymerase also has a strong 3'-5' exo-checking activity, which guarantees The fidelity is 1000 times that of Taq enzyme, which is higher than the fidelity of most current high-fidelity enzymes, which guarantees the high fidelity of DNA synthesis, and is very suitable for in vitro preparation of plasmids and complete gene assembly.   Store at 20°C. For long-term storage, please store below -70°C to avoid repeated freezing and thawing.
48.00
Article number:
N106-01/02
Number of views:
2813
Keywords:
phi29
dna
and
of
which
is
polymerase
max
the
323.00
Article number:
N105-01
The Klenow fragment (3´→5´exo-) is the N-terminal truncation of DNA polymerase I. It retains DNA polymerase activity, but loses 5´→3´ exonuclease activity; the enzyme has been mutated ( D355A, E357A) further removed its 3´→5´ exonuclease activity.   Simple and rapid isothermal reaction speeds up the preparation of plasmid samples for sequencing; High fidelity, strong chain switching activity, suitable for whole genome amplification   Store at -20℃
323.00
Article number:
N105-01
Number of views:
2233
Keywords:
fragment
the
activity
dna
exonuclease
polymerase
for
of
but
been
329.00
Article number:
N104-01
DNA polymerase I, the large fragment (Klenow fragment) is the protease hydrolysate of E. coli DNA polymerase I, with 5´→3´ DNA polymerase activity and 3´→5´ exonuclease activity, but 5 is missing ´→3´ Exonuclease activity. The Klenow fragment retains the high fidelity of the holoenzyme without degrading the 5´ end of DNA.   Simple and rapid isothermal reaction speeds up the preparation of plasmid samples for sequencing; High fidelity, strong chain switching activity, suitable for whole genome amplification    Store at -20℃
329.00
Article number:
N104-01
Number of views:
2096
Keywords:
fragment
the
of
activity
polymerase
dna
for
klenow
fidelity
581.00
Article number:
N103-01
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA. The enzyme not only catalyzes the connection between blunt ends or sticky ends, but also repairs single-strand cuts in double-stranded DNA, RNA, or DNA/RNA hybrids.   3' end with dA tail; Prepare probes with random primers; Random primer labeling   Store at -20℃
581.00
Article number:
N103-01
Number of views:
3747
Keywords:
t4
dna
ends
or
the
random
catalyzes
between
rna
double-stranded
169.00
Article number:
N102-01
T4 Polynucleotide Kinase can catalyze the transfer of the γ-phosphate group of ATP to the 5´-hydroxyl end and 3´-monophosphate nucleoside of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA). T4 Polynucleotide Kinase also has 3´-phosphatase activity, which hydrolyzes 3´-phosphate groups from the 3´ phosphate ends of oligonucleotides, deoxy 3´-monophosphate nucleosides and deoxy 3´-diphosphate nucleosides .   Prepare probes with random primers; Cut off the 3′ protruding end or fill in the 5′ protruding end to form a flat end   Store at -20℃
169.00
Article number:
N102-01
Number of views:
2411
Keywords:
t4
the
of
end
or
phosphate
polynucleotide
kinase
nucleosides
deoxy
165.00
Article number:
N101-01
In the presence of templates and primers, T4 DNA polymerase catalyzes the synthesis of DNA along the 5´→3´ direction. This enzyme also has 3´→5´ exonuclease activity, which is stronger than DNA polymerase I. Unlike DNA polymerase I, T4 DNA polymerase does not have 5´→3´ exonuclease activity.   Connect the sticky end or blunt end quickly in 5 minutes at room temperature; T/A cloning; dsDNA cut repair    Store at -20℃
165.00
Article number:
N101-01
Number of views:
2404
Keywords:
t4
dna
polymerase
the
of
end
in
exonuclease
at

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