VAHTSTM AmpSeq Cancer HotSpot Panel contains 207 primer pairs that can be used to amplify hotspot mutation regions of human tumor-related genes. The products cover a total of 50 proto-oncogenes and suppressor genes.
2800 common mutation sites of oncogenes. When this product is used in conjunction with VAHTSTM AmpSeq Library Prep Kit V2 (Vazyme #NA201), 20 μl multiple amplification system needs to add 10 μl of this Panel (that is, the concentration of this Panel is 2×), which can perform highly uniform multiplex amplification on the target area. Amplifier builds library, and provides VAHTSTM AmpSeq Adapters 1-96 for Illumina (Vazyme #NA111) and VAHTSTM AmpSeq Adapters 1-96 for Ion Torrent (Vazyme #NA121) for different sequencing platforms. The premixed primer panel saves researchers the time to design and prepare primers, and is suitable for starting DNA templates as low as 1ng and a variety of complex templates, such as FFPE samples and cfDNA. The fragment size of the amplified product using this product is between 111 bp-187 bp.
Store at -30 ~ -15℃
VAHTSTM AmpSeq Adapters for Ion Torrent is a special kit for the construction of Ion Torrent high-throughput sequencing platform library by AmpSeq technology. It can be used in conjunction with the AmpSeq library building kit to construct a multi-sample targeted sequencing DNA library suitable for the Ion Torrent platform. Kit NA121-01 contains 12 different barcode connectors from Adapter Barcode 1 to Adapter Barcode 12; Kit NA121-02 contains 12 different barcode connectors from Adapter Barcode 13 to Adapter Barcode 24; Kit NA121-03 contains Adapter Barcode 25 to Adapter Barcode 48 has a total of 24 different barcode connectors; kit NA121-04 contains Adapter Barcode 49 to Adapter Barcode 72, a total of 24 different barcode connectors; kit NA121-05 contains Adapter Barcode 73 to Adapter Barcode 96, a total of 24 different barcode connectors. All adapters provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.
Store at -30 ~ -15℃
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