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NGS Library Prep kits

Molecular Research

Drug Discovery

DNA Library Prep - for Illumina®

165.00
Article number:
N101-01
In the presence of templates and primers, T4 DNA polymerase catalyzes the synthesis of DNA along the 5´→3´ direction. This enzyme also has 3´→5´ exonuclease activity, which is stronger than DNA polymerase I. Unlike DNA polymerase I, T4 DNA polymerase does not have 5´→3´ exonuclease activity.   Connect the sticky end or blunt end quickly in 5 minutes at room temperature; T/A cloning; dsDNA cut repair   Store at -20℃
165.00
Article number:
N101-01
Number of views:
1000
Keywords:
301.00
Article number:
P507-02
301.00
Article number:
P507-02
Number of views:
1000
Keywords:
71.00
Article number:
P507-01
71.00
Article number:
P507-01
Number of views:
1000
Keywords:
0.00
Article number:
N611-02
0.00
Article number:
N611-02
Number of views:
1000
Keywords:
0.00
Article number:
N611-01
0.00
Article number:
N611-01
Number of views:
1000
Keywords:
0.00
Article number:
TD601
0.00
Article number:
TD601
Number of views:
1000
Keywords:
870.00
Article number:
NA102-01
VAHTSTM AmpSeq Cancer HotSpot Panel contains 207 primer pairs that can be used to amplify hotspot mutation regions of human tumor-related genes. The products cover a total of 50 proto-oncogenes and suppressor genes. 2800 common mutation sites of oncogenes. When this product is used in conjunction with VAHTSTM AmpSeq Library Prep Kit V2 (Vazyme #NA201), 20 μl multiple amplification system needs to add 10 μl of this Panel (that is, the concentration of this Panel is 2×), which can perform highly uniform multiplex amplification on the target area. Amplifier builds library, and provides VAHTSTM AmpSeq Adapters 1-96 for Illumina (Vazyme #NA111) and VAHTSTM AmpSeq Adapters 1-96 for Ion Torrent (Vazyme #NA121) for different sequencing platforms. The premixed primer panel saves researchers the time to design and prepare primers, and is suitable for starting DNA templates as low as 1ng and a variety of complex templates, such as FFPE samples and cfDNA. The fragment size of the amplified product using this product is between 111 bp-187 bp.   Store at -30 ~ -15℃
870.00
Article number:
NA102-01
Number of views:
1000
Keywords:
vahts
the
and
of
is
vahtstm
panel
ampseq
this
for
492.00
Article number:
ND617-01/02
VAHTS® Universal Plus DNA Library Prep Kit for Illumina is a DNA library construction kit developed for the Illumina® high-throughput sequencing platform. This kit combines DNA fragmentation, end repair, and dA tailing into one step. The product does not need to be purified. It is directly connected to adapters, library enrichment and sorting, and can convert 100 pg-1 μg template DNA into Illumina® Qualcomm Volume sequencing platform dedicated library. There is no need to mechanically interrupt the genome, which simplifies the library construction process and shortens the operation time, and is suitable for PCR and PCR-Free library construction. This kit is perfectly compatible with different sources and different input amounts of DNA, and only needs to adjust the fragmentation time according to the target insert size to obtain the desired fragment size library.   Store at -30 ~ -15℃
492.00
Article number:
ND617-01/02
Number of views:
3892
Keywords:
prep
文库
片段
dna
构建
兼容性
gdna
不同
模板
100
458.00
Article number:
ND608-01/02
458.00
Article number:
ND608-01/02
Number of views:
3698
Keywords:
428.00
Article number:
ND607-01/02
VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 is a library construction kit optimized for the Illumina® high-throughput sequencing platform. This kit can convert 100 pg-4 μg Input DNA into a dedicated library for the Illumina® high-throughput sequencing platform. As a new upgraded version, VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 greatly improves the library conversion rate and amplified library output through the overall improvement of the end repair module, the connection module and the library amplification module. PCR or PCR-Free library construction of various samples, and compatible with the targeted capture process. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.
428.00
Article number:
ND607-01/02
Number of views:
7747
Keywords:
vahts
the
library
and
kit
for
illumina
dna
of
construction
9999.00
Article number:
ND104-01
VAHTS Mate Pair Library Prep kit for Illumina is a kit optimized for Illumina high-throughput sequencing platform library construction. Using this reagent, DNA samples can be prepared into Illumina high-throughput sequencing platform-specific libraries. All reagents provided in the kit have undergone strict quality control and work verification, ensuring the stability and repeatability of library construction to the greatest extent. Using the non-gel recovery scheme, you can get a Mate Pair library with insert size between 2-15 kb, mainly concentrated in 3-5 kb. The mate pair library is obtained by the gel recovery scheme, and the size of the insert fragment depends on the size of the fragment obtained by the gel recovery.   It takes less time to build the library, and the library can be constructed in 75 minutes; More efficient linker connection efficiency, higher library conversion rate; Easier and faster operation, pre-mixing to reduce operating errors; Wider template compatibility range, can efficiently repair 100 pg-1 μg Input DNA; Wider sample compatibility, applicable to cfDNA, FFPE and fragmented DNA   Box1, Box2 and Box4 are stored at -20°C, and Box3 is stored at room temperature
9999.00
Article number:
ND104-01
Number of views:
2103
Keywords:
vahts
the
library
and
to
can
dna
in
is
size
75.00
Article number:
N616-01/02
VAHTSTM HiFi Amplification Mix is ​​a new high-fidelity PCR amplification master mix, suitable for PCR amplification of high-throughput sequencing libraries. The master mix is ​​based on VAHTSTM HiFi DNA Polymerase, which is a new generation of high-yield, high-fidelity DNA polymerase modified from Pfu DNA Polymerase. The sensitivity is greatly improved, and it has extremely high amplification efficiency and wide template adaptability. Expansion of output and plateau period. At the same time, its amplification mismatch rate is 1/52 of Taq DNA Polymerase and 1/6 of Pfu DNA Polymerase, ensuring the authenticity of library amplification. Coupled with a carefully optimized buffer system, VAHTSTM HiFi Amplification Mix can achieve low preference, wide template input range, high yield, and high stability amplification of high-throughput sequencing libraries. This product contains all the components required for PCR amplification, and contains library amplification primers for the Illumina sequencing platform. It is only necessary to add the library template to perform the reaction, and it is convenient to use; it contains a special protective agent and is stored for a long time or after repeated freezing and thawing. Can still maintain stable activity. The amplified product is blunt-ended and can be directly used for blunt-ended cloning.   Single-tube end repair, dA tailing reaction; End repair plus A takes less time, only 30 minutes; The reagents are in Mix form, compatible with automated library building equipment The template amount has a wider compatibility range, and can efficiently repair 100 pg-1 μg Input DNA; A wider range of sample types are compatible, suitable for cfDNA, FFPE and fragmented DNA    Store at -30 ~ -15℃
75.00
Article number:
N616-01/02
Number of views:
3079
Keywords:
vahts
and
the
is
amplification
of
dna
for
mix
it
9999.00
Article number:
N611-01/02
Amplification Mix2 is a new type of high-fidelity PCR amplification master mix, suitable for PCR amplification of high-throughput sequencing libraries. The master mix is ​​based on VAHTSTM Super-Fidelity DNA Polymerase, which is a new generation of high-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and its amplification mismatch rate is 1/52 of Taq DNA Polymerase is 1/6 of Pfu DNA Polymerase. With a carefully optimized reaction buffer, Amplification Mix2 can achieve low-preference, high-efficiency, and high-stability amplification of high-throughput sequencing libraries. This product contains all the components required for PCR amplification, and only needs to add template primers to perform the reaction, which is convenient to use; it also contains a special protective agent to maintain stable activity after long-term storage or repeated freezing and thawing. The amplified product is blunt-ended and can be directly used for blunt-ended cloning.   Contains 207 primer pairs that can be used to amplify hot mutation regions of human tumor-related genes; The products cover a total of 2800 common mutation sites of 50 proto-oncogenes and tumor suppressor genes   Store at -20°C, valid for one year
9999.00
Article number:
N611-01/02
Number of views:
1853
Keywords:
amplification
of
is
and
dna
the
to
for
contains
184.00
Article number:
N802-01/02
VAHTSTM DNA Adapters set1/2 for Illumina® is a special kit for Illumina high-throughput sequencing platform library construction, suitable for the construction of multi-sample DNA library of Illumina high-throughput sequencing platform. The set1 kit (N801) contains 12 different Index adapters for DNA Adapter 1-12; the set2 kit (N802) contains 12 different Index adapters for DNA Adapter 13-27. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   All components are stored at -20°C, valid for one year
184.00
Article number:
N802-01/02
Number of views:
2465
Keywords:
vahts
for
the
dna
kit
adapters
illumina
library
of
construction
184.00
Article number:
N801-01/02
VAHTSTM DNA Adapters set1/2 for Illumina® is a special kit for Illumina high-throughput sequencing platform library construction, suitable for the construction of multi-sample DNA library of Illumina high-throughput sequencing platform. The set1 kit (N801) contains 12 different Index adapters for DNA Adapter 1-12; the set2 kit (N802) contains 12 different Index adapters for DNA Adapter 13-27. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   All components are stored at -20°C, valid for one year
184.00
Article number:
N801-01/02
Number of views:
3188
Keywords:
vahts
for
the
dna
kit
adapters
illumina
library
of
construction
287.00
Article number:
N204-01/02
VAHTSTM Universal Adapter Ligation Module for Illumina® is a connection module optimized for Illumina high-throughput sequencing platform library construction. Use this kit Linkers containing dT protrusions can be efficiently connected to the end of repair products containing phosphate groups at the 5'end and dA protrusions at the 3'end. Compared with the conventional method, the VAHTSTM Universal Adapter Ligation Module for Illumina® uses a one-tube reaction. After the fragmented DNA is processed by the VAHTSTM Universal End Preparation Module for Illumina® (Vazyme #N203), the adapter ligation reaction can be carried out directly without unnecessary products. Purification step. Therefore, the minimum requirement of the starting DNA template is significantly reduced, and the experiment time is shortened. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.    Single sample library construction time is less than 90 minutes;Single-tube enzymatic reaction, fragmentation and linker reaction are carried out at the same time, without using mechanical methods (ultrasound, atomization, etc.) for fragmentation;Special polymerase for library amplification, coupled with a carefully optimized reaction buffer, ensures the maximum efficiency and uniformity of library amplification;The minimum amount of DNA for library construction is only 1 ng;Classified according to the amount of starting DNA, a total of 3 kits with different specifications are provided; Provide 96 double-ended index combinations     Store at -20°C  
287.00
Article number:
N204-01/02
Number of views:
2000
Keywords:
vahts
the
for
of
is
library
and
dna
module
at
179.00
Article number:
N203-01/02
VAHTSTM Universal End preparation Module for Illumina® is an end repair module optimized for Illumina high-throughput sequencing platform library construction. Using this kit can efficiently repair the ends of 100 pg – 1 μg of fragmented DNA. The repair product contains phosphate groups at the 5'end and dA overhangs at the 3'end. Compared with conventional library building methods, VAHTSTM Universal End preparation Module for Illumina® uses a one-step reaction process, which combines the end repair and dA tailing steps, while reducing the reaction time of each step, thus significantly reducing the minimum starting DNA template The amount of demand reduces the time-consuming experiment. The reagents are in the form of Mix, which makes pipetting operations more convenient and compatible with automated library building equipment. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   Efficient connection;No purification is required, and the ligation reaction can be carried out directly in the dA-Tailing reaction product;Compatible with 100 pg-1 μg Input DNA   Store at -20°C
179.00
Article number:
N203-01/02
Number of views:
1862
Keywords:
vahts
the
and
of
end
library
reaction
repair
in
module
209.00
Article number:
TD202
TruePrepTM Index Kit V2 for Illumina® is a dedicated kit for TruePrepTM DNA Library Prep Kit V2 for Illumina®. It contains 8 types of N5XX and 12 types of N7XX, which can be used to prepare 96 different double-ended Index libraries. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   It has a high success rate for complex plant templates; The library construction process is the same as Illumina TruSeq RNA Sample Preparation Kit v2; Optimized large fragment library construction program; Sequencing data is highly consistent with Illumina reagents   All components are stored at -30°~ -15°C.
209.00
Article number:
TD202
Number of views:
5070
Keywords:
TruePrep Index Kit V2 for Illumina®
TD202
1270.00
Article number:
N302/N303
VAHTSTM TurboDNALibraryPrepKitforIllumina®专用接头引物模块VAHTSTM MultiplexOligosset1/set2forIllumina®VAHTSTM MultiplexOligosset1/set2forIllumina®是Illumina高通量测序平台文库构建专用试剂盒。试剂盒中包含VAHTSAdapterforIllumina,VAHTSU
1270.00
Article number:
N302/N303
Number of views:
2053
Keywords:
VAHTS Multiplex Oligos set1/set2 for Illumina®
N302/N303
669.00
Article number:
N805/N806/N807/N808
VAHTSTM DNA Adapters set3-set6 for Illumina® are dedicated kits for Illumina high-throughput sequencing platform library construction, suitable for Illumina high-throughput sequencing platform multi-sample DNA library construction. The set3 kit (N805) contains 24 different indexes from DNA Adapter 96-01 to DNA Adapter 96-24; the set4 kit (N806) contains 24 different indexes from DNA Adapter 96-25 to DNA Adapter 96-48 Adapter; set5 kit (N807) contains 24 different Index adapters from DNA Adapter 96-49 to DNA Adapter 96-72; set6 kit (N808) contains 24 types from DNA Adapter 96-73 to DNA Adapter 96-96 Different Index connectors. All adapters provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.    Store at -20°C, valid for one year. Do not store this product in an environment where the temperature is higher than room temperature
669.00
Article number:
N805/N806/N807/N808
Number of views:
3573
Keywords:
vahts
dna
adapter
the
to
kit
from
different
contains
24
9999.00
Article number:
TD203
TruePrepTM Index Kit V3 for Illumina® is a dedicated kit for TruePrepTM DNA Library Prep Kit V2 for Illumina®. It contains 16 kinds of N6XX and 24 kinds of N8XX, which can be used for the preparation of 384 different double-ended Index libraries. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   It has a high success rate for complex plant templates; The library construction process is the same as Illumina TruSeq RNA Sample Preparation Kit v2; Optimized large fragment library construction program; Sequencing data is highly consistent with Illumina reagents   All components stored at -20°C
9999.00
Article number:
TD203
Number of views:
2604
Keywords:
TD203
145.00
Article number:
TD601
The amplification part used in the DNA library building kit TruePrep DNA Library Prep Kit V2 for Illumina® contains a polymerase dedicated to library amplification and is equipped with a carefully optimized reaction buffer to ensure the highest efficiency and efficiency of library amplification. Uniformity, high efficiency and stable amplification can be achieved for templates with different starting amounts, and only 1 ng starting DNA is required. Can be used with or supplement TruePrep DNA Library Prep Kit V2 for Illumina® (TD501/TD502/TD503).   easy to use; Compatible with multiple types of libraries; Very wide range of template starting amount; Excellent amplification speed; Excellent stability
145.00
Article number:
TD601
Number of views:
2678
Keywords:
TD601
870.00
Article number:
NA102-01
VAHTSTM AmpSeq Cancer HotSpot Panel contains 207 primer pairs that can be used to amplify hotspot mutation regions of human tumor-related genes. The products cover a total of 50 proto-oncogenes and suppressor genes. 2800 common mutation sites of oncogenes. When this product is used in conjunction with VAHTSTM AmpSeq Library Prep Kit V2 (Vazyme #NA201), 20 μl multiple amplification system needs to add 10 μl of this Panel (that is, the concentration of this Panel is 2×), which can perform highly uniform multiplex amplification on the target area. Amplifier builds library, and provides VAHTSTM AmpSeq Adapters 1-96 for Illumina (Vazyme #NA111) and VAHTSTM AmpSeq Adapters 1-96 for Ion Torrent (Vazyme #NA121) for different sequencing platforms. The premixed primer panel saves researchers the time to design and prepare primers, and is suitable for starting DNA templates as low as 1ng and a variety of complex templates, such as FFPE samples and cfDNA. The fragment size of the amplified product using this product is between 111 bp-187 bp.     Store at -30 ~ -15℃
870.00
Article number:
NA102-01
Number of views:
1908
Keywords:
vahts
the
and
of
is
vahtstm
panel
ampseq
this
for
609.00
Article number:
NA111-01/02/03/04/05
VAHTSTM AmpSeq Adapters for Illumina is a dedicated kit for the construction of AmpSeq technology for Illumina high-throughput sequencing platform libraries. Used in conjunction with the AmpSeq library construction kit, it can construct a multi-sample targeted sequencing DNA library suitable for the Illumina platform. Kit NA111-01 contains 12 different index connectors from Adapter 1 to Adapter 12; Kit NA111-02 contains 12 different index connectors from Adapter 13 to Adapter 24; Kit NA111-03 contains 24 types from Adapter 25 to Adapter 48 Different index adapters; kit NA111-04 contains 24 different index adapters from Adapter 49 to Adapter 72; kit NA111-05 contains 24 different index adapters from Adapter 73 to Adapter 96. All adapters provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   Store at -30 ~ -15℃
609.00
Article number:
NA111-01/02/03/04/05
Number of views:
1939
Keywords:
VAHTS AmpSeq Adapters for Illumin
VAHTS AmpSeq Adapters for Illumin
209.00
Article number:
TD204/TD205/TD206/TD207
TruePrepTM Index Kit V4 for Illumina® is a dedicated kit for TruePrepTM DNA Library Prep Kit V2 for Illumina® (vazyme#TD501-503), including 1 DNA Adapter N101 (i5) and 96 kinds of DNA Adapters N9XX (i7) can be used for the preparation of 96 different single-ended index tag libraries. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.    All components stored at -20°C
209.00
Article number:
TD204/TD205/TD206/TD207
Number of views:
2726
Keywords:
illumina
for
kit
the
dna
and
of
96
all
index
1675.00
Article number:
N321/N322
VAHTSTM Multiplex Oligos set 4 for Illumina® is a special kit for Illumina high-throughput sequencing platform library construction. The kit contains VAHTS Adapter-S for Illumina, 8 types of VAHTS i5 PCR Primers and 12 types of VAHTS i7 PCR Primers, together with VAHTS Universal DNA Library Prep Kit for Illumina® V2 (Vazyme #ND606), which can be used to construct up to 96 different types The combined double-ended Index tag library. At the same time, in conjunction with VAHTSMultiplex Oligos set 5 for Illumina®, it can be used to construct up to 384 different combinations of double-ended Index marker libraries. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   All components are stored at -20°C. Do not store this product in an environment where the temperature is higher than room temperature.
1675.00
Article number:
N321/N322
Number of views:
2935
Keywords:
VAHTS Multiplex Oligos Set 4/5 for Illumina®
N321/322
179.00
Article number:
NA205-01/02
VAHTSTM AmpSeq Multi-PCR Module V2 is an amplification reagent developed and designed on the basis of ultra-multiplex PCR technology. It is suitable for multiple amplification of 1-100 ng of gDNA, FFPE DNA, cfDNA and other templates. The optimized multiple amplification reagents include enzymes, dNTPs and reaction buffers required for amplification, which reduce pipetting operations during the experiment and make the results more stable. It can help researchers and testers to complete the ultra-high quality easily, quickly and with high quality. Multiplex PCR amplification is suitable for downstream targeted amplicon library construction and other experiments. This amplification reagent has undergone strict quality control and functional verification to ensure the stability and repeatability of the reagent to the greatest extent.   The operation is quick and easy: from DNA to library amplification as low as 5 hours, manual operation time is less than 1.5 hours, and no purification steps are in between; Compatible with multiple types of samples: compatible with genomic DNA, cfDNA, FFPE DNA; Very high on-target ratio: the ratio is close to 100%; Excellent data uniformity: >95%; Very low template starting amount: starting DNA template amount as low as 1 ng   Store at -30 ~ -15℃
179.00
Article number:
NA205-01/02
Number of views:
2073
Keywords:
VAHTS AmpSeq Multi-PCR Module V2
NA205-01/02

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这是描述信息

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