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NGS Library Prep kits

Molecular Research

Drug Discovery

Genome Editing

196.00
Article number:
TR102-01/02
T7 RNA ploymerase can recognize DNA templates with T7 promoters, and use four NTPs as substrates to synthesize RNA by in vitro transcription. The T7 RNAi Transcription Kit is an optimized version designed for the transcription of double-stranded RNA based on the T7 High Yield RNA Transcription Kit. It can be used to transcribe 21 bp siRNA and long fragment dsRNA. The purified transcript can be used in RNAi experiments mediated by cationic liposomes, calcium phosphate co-precipitation, electroporation, DEAE-dextran, and microinjection. In general, one reaction can produce 20-80 µg of RNA.   Can transcribe siRNA and long dsRNA; The yield is as high as 20-80 μg; RNA magnetic beads can quickly and efficiently purify transcription products   Box 1 is stored at -20°C, and Box 2 is stored at 4°C
196.00
Article number:
TR102-01/02
Number of views:
3718
Keywords:
T7 RNAi Transcription Kit
174.00
Article number:
TR101-01/02
T7 High Yield Transcription Kit has optimized the transcription reaction system. T7 RNA Polymerase synthesizes RNA complementary to one strand of DNA from the downstream of the template DNA T7 promoter. It is simple and fast to obtain a large number of RNA molecules, which can be in the substrate during transcription. Add modified nucleotides to prepare biotin or dye-labeled RNA. This kit can produce 150-200 μg of RNA with 0.5 μg of template input. The RNA synthesized by transcription can be used for research such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. Aspects of downstream applications.    Optimized reaction system; Each reaction produces up to 150-200 μg of RNA product   Store at -20℃
174.00
Article number:
TR101-01/02
Number of views:
5135
Keywords:
T7 High Yield RNA Transcription Kit
54.00
Article number:
EN303-01/02
T7 Endonuclease I recognizes and cuts incompletely matched DNA, cross-shaped structure DNA, Holiday structure or DNA bifurcation points, heterologous DNA; at the same time, it can cut double-stranded DNA with cutting sites at a lower speed. The enzyme cleaves the first, second or third phosphodiester bond at the 5'end of the mismatch site. This product is a high-purity active protein expressed and purified in Escherichia coli after the recombinant T7 endonuclease I gene has been cloned. It can be used for gene mutation, SNP, TALEN or CRISPR/Cas9 mutant detection, identification of mismatched DNA, and decomposition of four Directional cross DNA or branched DNA; detection of heterodimer or cut DNA; or used for random cutting of linear DNA for Shot-gun cloning.   High detection sensitivity; Products can be directly detected by electrophoresis   Store at -20°C
54.00
Article number:
EN303-01/02
Number of views:
4176
Keywords:
T7EndonucleaseI
28.00
Article number:
EN301-01/02
Cas9 Nuclease is an RNA-guided sequence-specific double-stranded DNA endonuclease. The guide RNA recognizes the target site by being complementary to the target sequence and brings Cas9 Nuclease to the target DNA. Cas9 Nuclease has two cleavage active centers, which respectively cut the two strands of target DNA to produce DNA double-strand breaks. The cutting site is three bases away from NGGPAM in the targeted region. This product is a high-purity Cas9 active protein expressed and purified in E. coli after the Cas9 gene of Streptococcus pyogenes has been cloned.   High purity and high activity Cas9 protein; Convenient for in vitro sgRNA efficiency verification   Store at -20°C
28.00
Article number:
EN301-01/02
Number of views:
3926
Keywords:
Cas9Nuclease

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