VAHTSTM Serum/Plasma Circulating DNA Kit is a superparamagnetic purification technology based on magnetic beads. It is a DNA extraction kit optimized for the enrichment of short fragments of free DNA in serum/plasma samples. Superparamagnetic silica nanomagnetic beads have a degree of dispersion. High, large surface area, good adsorption specificity, etc., efficiently bind and enrich DNA fragments.
This kit is used to extract high-quality free DNA from serum and plasma samples with a flexible starting range (200 μl~2 ml). The operation is simple and fast, and there is no toxic organic solvent in the whole process. Targeted optimization for low molecular weight nucleic acids, it has the advantages of no preference for DNA extraction, high extraction yield, high nucleic acid purity, and strong reproducibility. The obtained DNA can be directly used in experiments such as quantitative PCR and second-generation sequencing. Compatible with manual extraction and automatic extraction processes, and compatible with cfDNA blood collection tubes without free aldehyde groups, such as Streck Cell-Free DNA BCT® or VAHTSTM. Free DNA storage blood collection tubes or ordinary EDTA blood collection tubes obtain plasma samples.
Compatible with manual extraction and automatic extraction procedures"
Box1, store at 2~8°C; the dissolved Proteinase K must be stored at -20°C; Box2, store at room temperature
Plasma free DNA (Cell-free DNA) is an important test sample for non-invasive prenatal diagnosis and tumor diagnosis, and its role and importance are becoming increasingly apparent. In untreated plasma, cfDNA is easily degraded by nucleases. At the same time, the large amount of genomic DNA produced by the death of white blood cells in the plasma will also seriously interfere with downstream applications. Therefore, the storage time of whole blood used for cfDNA extraction should usually not exceed 12 hours. , Which greatly limits the application of cfDNA in clinical diagnosis. VAHTSTM blood collection tubes for free DNA preservation are independently developed by Novartis Biotechnology Co., Ltd., which are specially used for blood collection and preservation tubes for long-term stable preservation of free DNA in whole blood. This product contains a non-toxic and non-free aldehyde protective agent that can effectively maintain the integrity of white blood cells in plasma and avoid the release of genomic DNA. At the same time, the nuclease inhibitor in the product ensures that free DNA is not degraded, thereby achieving long The purpose of stably preserving free blood DNA. The blood drawn with this product can ensure stable storage of free DNA at room temperature for 14 days, and at the same time can withstand continuous shaking for 24 hours in the temperature range of 4~30℃, which greatly facilitates the storage and transportation of samples.
The extracted cfDNA has good coverage and no preference, suitable for the second-generation sequencing database construction experiment;
Empty tube storage: It can be stored stably at 18~30℃, and can not be frozen; After blood collection: After blood collection, whole blood can be stored stably at 6~30℃ for no more than 14 days. The release of leukocyte genome is slower when stored at 4°C, but the potential risk of hemolysis is higher than when stored at room temperature.
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