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NGS Library Prep kits

Molecular Research

Drug Discovery

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2 × Phanta Max Master Mix

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specificity promoting factors, and plateau uninhibitory factors, which have greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Increased. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can Effectively amplify fragments up to 10 kb. The mismatch rate is 1/53 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added with two monoclonal antibodies that can inhibit its 5’→3’ polymerase activity and 3’→5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added in the system allows 2 × Master mix to maintain stable activity after repeated freezing and thawing. The amplified product is blunt-ended and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).   Super fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are less than 5 mismatches; Amplify faster: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: simple templates such as plasmids and lambda DNA can be amplified up to 40 kb, cDNA can be amplified up to 10 kb, and genome can be amplified up to 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.   Store at -20°C
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56.0
Number of views:
15037
Product serial number
P515-01/02/03
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Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specificity promoting factors, and plateau uninhibitory factors, which have greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Increased. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can Effectively amplify fragments up to 10 kb. The mismatch rate is 1/53 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added with two monoclonal antibodies that can inhibit its 5’→3’ polymerase activity and 3’→5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added in the system allows 2 × Master mix to maintain stable activity after repeated freezing and thawing. The amplified product is blunt-ended and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).

 

Super fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are less than 5 mismatches;
Amplify faster: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments;
Longer amplification: simple templates such as plasmids and lambda DNA can be amplified up to 40 kb, cDNA can be amplified up to 10 kb, and genome can be amplified up to 20 kb;
Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.

 

Store at -20°C

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